摘要
目的:观察电针处理对不同状态大鼠大脑皮层ATP结合盒式蛋白和基因表达的影响,探索其对正常和病理状态下血脑屏障相关功能蛋白的影响。方法:SD大鼠分为空白组、空白电针组、抑郁组和抑郁电针组。采用慢性应激刺激法复制抑郁大鼠模型。空白电针组及抑郁电针组电针"百会""哑门",连续波,频率2Hz,强度1mA,每次20min,每日1次,连续电针14d。通过蛋白印迹法和逆转录多聚酶链反应技术对大脑皮层多药耐药基因(Mdr)1a、Mdr 1b,多药耐药相关蛋白(Mrp)1、Mrp 4、Mrp 5、乳腺癌耐药蛋白(Bcrp)和基因表达进行检测。结果:与空白组比较,抑郁组未见这些蛋白和基因表达的显著改变(P>0.05)。电针处理能显著减少正常和抑郁大鼠大脑皮层Mdr 1a的蛋白和基因表达(P<0.05,P<0.01),但电针对其它蛋白和基因的表达未见显著影响(P>0.05)。结论:无论正常状态还是抑郁状态大鼠,电针"百会""哑门"均可显著抑制其皮层P-糖蛋白的表达,这可能是电针开放血脑屏障的重要途径。
Objective To observe the effect of electroacupuncture （EA） on the expression of blood-brain barrier （BBB）-related functional protein, ATP-binding cassette protein and gene in the cerebral cortex of depression rats. Methods Forty SD rats were equally and randomly divided into control group, EA group, depressive （model） group and depression ＋ EA group. The depression model was established by chronic unpredictable mild stress. EA （2 Hz, 1 mA） was applied to ＂Baihui＂（GV 20） and ＂Yamen＂（GV 15） for 20 min, once daily for 14 days. The expression levels of multidrug resistance （Mdr） la, Mdr lb, multidrug resistance-associated protein （Mrp）1, Mrp 4, Mrp 5, and breast cancer resistance protein （Bcrp） gene and proteins in the cerebral cortex tissue were detected by RT-POR semi-quantitative analysis and Western blot, respectively. Results In comparison with the control group, no obvious changes were found in the expression levels of cerebral Mdr la, Mdr lb, Mrp 1, Mrp 4, Mrp 5 and Bcrp genes and proteins in the model group （P〉0.05）, and only cerebral Mdr la gene and protein expression levels in the EA group were evidently down-regulated （P〈0.01, P〈0.05）. Compared with the model group, only cerebral Mdr la gene and protein expression levels were obviously decreased in the depression＋ EA group （P〈0.01, P〈0.05）. No signifi- cant changes were found in the Mdr lb, Mrp 1, Mrp 4, Mrp 5 and Bcrp genes and proteins after EA intervention in depression rats （P〉0.05）. Conclusion EA of GV 20 and GV 15 can obviously down-regulate the expression of cerebral Mdr la in depression rats which might be involved in the opening activity of blood-brain barrier.
出处
《针刺研究》
CAS
CSCD
北大核心
2013年第4期286-290,333,共6页
Acupuncture Research
基金
国家自然科学基金青年基金(81001651
81102634)
南京中医药大学针药结合省部共建教育部重点实验室开放课题(KJA 200905)
关键词
抑郁
电针
大脑皮层
ATP结合盒式转运蛋白
血脑屏障
Depression
Electroacupuncture
Cerebral cortex
ATP-binding cassette transporter
Blood-brainbarrier
