摘要
目的:建立利用自微乳浸提串联浊点浓缩分离蛇床子中香豆素类化合物的工艺。方法:以蛇床子素溶解量为指标,通过单因素试验筛选油相、乳化剂、助乳化剂,利用伪三元相图优化自微乳处方;以蛇床子素和总香豆素提取量为指标,采用U5(54)均匀试验考察浸提温度、时间、液固比和超声功率对提取工艺的影响,通过浊点浓缩分离自微乳提取液中香豆素类成份。结果:优选的微乳液处方为[聚乙二醇辛基苯基醚-正丁醇(5∶1)]-油酸乙酯-水(2.5∶0.5∶7);最优工艺参数为液固比20∶1,提取温度50℃,超声功率280 W,浸提时间60 min,蛇床子素和总香豆素的提取率分别达83.2%,91.19%;利用浊点浓缩方法处理自微乳提取液,油层中蛇床子素和总香豆素的回收率分别达90.33%,78.91%。结论:该工艺简单易行,适用于蛇床子活性成分的提取和浓缩分离。
Objective：To establish a process for separation and extraction coumarins from Cnidii Fructus by cloud-point preconcentration using self-microemulsion as solvent.Method：With dissolved amount of osthole as index,single factor tests were adopted to screen oil phase,emulsifier and co-emulsifier,microemulsion formulation was optimized by pseudo-ternary phase diagrams;Taking extraction amount of osthole and total coumarins as index,solid-liquid ratio,ultrasonic power,extraction temperature and time was investigated by U5（54） uniform test.To separate coumarins from self-microemulsion extract by cloud-point preconcentration.Result：Proportion of optimized prescription was [octyl polyethylene glycol phenyl ether-butyl alcohol（5：1） ] ethyl oleate-water（2.5 ：0.5 ：7）;Optimum technology parameters were as follows：extraction temperature of 50 ℃,extraction time 60 min,solid-liquid ratio of 20：1,ultrasonic power 280 W.Under these conditions,yield of osthole was 83.2%,total coumarins was 91.19%.After self-microemulsion extract treated by cloud-point preconcentration method,recovery of osthole and total coumarins in oil reservoir reached 90.33% and 78.91%,respectively.Conclusion：This optimized technology was simple and feasible,it was suitable for extraction and separation of active ingredients from Cnidii Fructus.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第17期1-4,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东高校优秀青年创新人才培养计划项目(LYM10092)
关键词
蛇床子
自微乳
浊点萃取
均匀设计
Cnidii Fructus
self-microemulsion
cloud-point extraction
uniform design
