摘要
目的:探讨微乳技术与闪式提取结合应用于丹参脂溶性成分提取的适用性。方法:以丹参酮ⅡA的提取率作为丹参脂溶性成分的指标,分别比较不同提取方式和不同溶剂对丹参中丹参酮ⅡA提取率的影响;采用正交设计,对丹参微乳闪式提取工艺的影响因素进行考察。结果:以微乳为溶剂,闪式提取可在数分钟内提取出丹参中80%～90%的丹参酮ⅡA;工艺条件中提取次数和微乳的稀释倍数对丹参酮ⅡA的提取率有非常显著影响。结论:微乳技术结合闪式提取应用于中药丹参有效成分的提取具有高效、快速的特点,应用前景良好。
Objective：To study the feasibility of extracting salvia miltiorrhiza by smashing tissue extraction and microemulsion technology.Method：To measure the content of tanshinon ⅡA in salvia miltiorrhiza microemulsion extracting solution by HPLC.Making microemulsion as a solvent to extract salvia miltiorrhiza by smashing tissue extraction,heating countercurrent extraction,ultrasonic extraction and compare the extraction rate of tanshinonⅡA.U-sing orthogonal design to analyze on effect factors of the smashing tissue extraction.Result：The extraction rate of tanshinon ⅡA is 80%～90% by smashing tissue extraction and microemulsion technology.Extraction frequency and microemulsion dilution multiple of smashing tissue extraction have very significant impact on the extraction rate of tanshinon ⅡA.Conclusion：Combination smashing tissue extraction with microemulsion technology to extract the active compounds of salvia miltiorrhiza has efficient and fast characteristics and better application prospect.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第2期15-17,20,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
"重大新药创制"科技重大专项(2009ZX09301)
(2009ZX09308)
中国中医科学院自主选题项目(Z02094)
关键词
闪式提取
微乳
丹参
丹参酮ⅡA
smashing tissue extraction
microemulsion
salvia miltiorrhiza
tanshinon ⅡA
