摘要
[目的 ]研究中药瑞香素在体外和体内的杀裂殖体作用。 [方法 ]在恶性疟原虫FCCl株常规体外培养中测试瑞香素杀裂殖体活性 ,并按“四天抑制性试验”在感染伯氏疟原虫ANKA株的小鼠中测定不同剂量瑞香素的体内抗疟活性。 [结果 ]体外试验中瑞香素在 1～ 10 μmol L剂量范围内有明显杀灭裂殖体作用 ,而体内试验中按D4减虫率与感染鼠在 30d内的平均生存天数评价 ,5 0或 10 0mg kg .d- 1 × 4d瑞香素灌胃以及 10 ,5 0或 10 0mg kg.d- 1 × 4d瑞香素腹腔注射给药在伯氏鼠疟原虫ANKA株感染鼠中的抗疟作用与 10mg kg .d- 1 × 4d氯喹 (CQ)灌胃的疗效相似。 [结论 ]瑞香素在体外和体内均有一定的杀裂殖体作用。
Objective] To investigate the in vitro and in vivo schizontocidal activity of daphnetin. [Methods] Schizontocidal activity of daphnetin was tested using an in vitro assay based on the routine in vitro cultivation of P. falciparum FCC 1 strain. The in vivo antimalarial effects of daphnetin at various dosages were assessed in mice infected with P b.erghei ANKA according to “4-day suppress assay”. [Results] In vitro ,daphnetin exhibited potent schizontocidal activity comparable to chloroquine(CQ) at the dose range of 1～10 μmol/L. In vivo ,50 or 100 mg/kg.d -1 ×4 d daphnetin i.g. and 10,50 or 100 mg/kg.d -1 ×4 d dephnetin i.p. showed antimalarial efficacy comparable to CQ 10 mg/kg.d -1 ×4 d i.g. in mice infected with P. berghei ANKA, evaluated by both the reduction rate of parasitemia on D 4 and the average surviving days in 30 days. [Conclusion]Daphnetin displays certain schizontocidal activity both in vitro and in vivo .
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2000年第4期204-206,共3页
Chinese Journal of Parasitology and Parasitic Diseases
基金
中国预防医学科学院基金
关键词
瑞香素
恶性疟原虫
杀裂殖体活性
daphnetin,Plasmodium falciparum,Plasmodium berghei, schizontocidal activity
