摘要
目的 :探讨阿霉素诱导卵巢癌细胞凋亡的规律。方法 :以不同浓度的阿霉素作用于人卵巢癌细胞株SKOV3,用光镜、电镜、TUNEL法和DNA凝胶电泳法检测确定细胞凋亡 ,以流式细胞仪定量分析凋亡和细胞周期特异性。结果 :大剂量 ( 2 5～10 0 μg/ml)长时间 ( 3～ 72h)的阿霉素作用可引起卵巢癌细胞坏死和凋亡 ,较小剂量( 0 .5μg/ml)的阿霉素连续作用 2 4、4 8、72h ,诱导细胞凋亡发生率分别为 4 0 .0 1%、65.38%和 77.65% ,并使细胞发生G1期阻滞。结论 :阿霉素能导致卵巢癌细胞DNA损伤 ,发生G1期阻滞并诱导凋亡是其抗肿瘤的机制之一。
Objective: To explore the effect of apoptosis induced by various concentration of doxorubicin at different time in ovarian carcinoma cell line SKOV3 in vitro. Methods: Apoptosis were evaluated by light and electron microscopy. DNA agarol gel electrophoresis, terminal deoxynucleotidyl transferase mediated dUTP in situ nick end labeling. The quantitative of apoptosis and cell's cycle specificity anaysis were measured by flow cytometry. Results: Ovarian carcinoma cells were treated with higher concentration doxorabicin(25-100μg/ml)at 3～72 h could induce necrosis and apoptosis. The lower concentration doxorubicin(0.5μg/ml)could induce mainly ovarian carcinoma cells apoptosis in G 1 phase. Conclusions: Doxorubicin can damage ovarian carcinoma cell's DNA,induct the cells G 1 phase arrest and induce cells apoptosis. It is one of the mechanism of anti-cancer chemotherapy of doxorubicin.
出处
《现代妇产科进展》
CSCD
2000年第5期338-340,共3页
Progress in Obstetrics and Gynecology
关键词
阿霉素
卵巢肿瘤
细胞凋亡
流式细胞术
Doxorubicin
Ovarian neoplasms
Cell apoptosis
Flow cytometry
