摘要
目的 探讨化疗药物顺铂 (CDDP)对口腔鳞癌细胞凋亡及细胞周期特异性的诱导作用。方法 选用人舌鳞状细胞癌细胞系Tca8113为研究对象 ,应用倒置显微镜、荧光显微镜、透射电镜、流式细胞仪等方法进行观察。结果 CDDP作用Tca8113细胞后 ,细胞增殖变慢 ,增殖指数下降 ;细胞变小、变圆 ,失去贴壁性 ,从附着处脱落。DNA染色后荧光显微镜下观察 ,细胞核呈现橙红色、半月形、不规则块状等凋亡形态变化。透射电镜观察 ,细胞固缩 ,染色质浓聚边缘化 ,凋亡小体形成 ;流式细胞仪测定显示细胞周期发生明显改变 ,细胞分裂阻滞在S期。结论 CDDP能诱导Tca8113细胞凋亡 ,其作用有时间依赖性 ;
Objective Evaluating the effect of apoptosis induced by cisplatin in human oral squamous cell carcinoma cells and their cycle specificity. Methods Human tongue squamous cell carcinoma cell line Tca8113 was used in vitro as a study object. Morphological features of apoptotic cell induced by cisplatin were observed by using of microscope, fluorescence microscope and transmission electronic microscope (TEM). The apoptotic rates were analyzed by DNA fluorescence staining assay and TEM observation. The cell cycle specificity was measured by using flow cytometry. Results The cell proliferation of Tca8113 cells was inhibited by cisplatin and their proliferation index decreased. Cells treated with cisplatin became smaller, rounded and then disattached. Red nuclear fragmentation with condensed chromatin was observed under fluorescence staining assay. Condensation and crack of nucleus and apoptotic bodies appeared in apoptotic Tca8113 cells observed under TEM. Cell cycle of Tca8113 cells was apparently arrested at S by cisplatin.Conclusion Apoptosis was induced by cisplatin in Tca8113 cells, and it may be an important mechanism of SCC killed by cisplatin.
出处
《上海口腔医学》
CAS
CSCD
2000年第3期161-163,共3页
Shanghai Journal of Stomatology
基金
上海市口腔医学研究所基金!(9907)
关键词
癌
舌
鳞状细胞
顺铂
凋亡
治疗
Neoplasm
Squamous Cell
Tongue
Cisplatin
Apoptosis
