摘要
目的观察顺铂和阿霉素联合作用对人舌鳞癌Tca8113细胞生长的抑制作用，以及对细胞周期和凋亡的影响。方法不同浓度顺铂、阿霉素及两者联合作用于人舌鳞癌Tea8113细胞，用MTT法观察细胞增殖情况以及药物对细胞生长的抑制效应。用流式细胞仪观察细胞凋亡和细胞周期。结果顺铂（r=0．538，P〈0．01）和阿霉素（r=0．642，P〈0．01）呈剂量和时间依赖性抑制Tca8113细胞生长。单独用1．25μmol／L阿霉素作用24h，对Tca8113细胞的生长无显著影响。不同浓度顺铂（2～10mg／L）单独作用24h，仅10mg／L浓度可显著抑制Tca8113细胞的生长（P〈0．01）。1．25μmol/L阿霉素与顺铂联合应用24h后，4、6、8、10mg／L顺铂均可显著抑制Tca8113细胞生长，且与相应浓度的单独用药组比，差异均有统计学意义（均为P〈0．001）。1．25μmol／L阿霉素、6mg／L顺铂以及阿霉素1．25μmol／L＋顺铂6mg／L均显著改变G0～G1期（50．00±0．32％，43．53±2．026％，50．77±3．83％）和S期（36．5±1．05％，40．8±2．52％，30．87±2．65％）细胞百分比（均为P〈0．01），联合用药的作用较单一用药组更显著（P〈0．05）。上述药物对细胞凋亡的作用均不明显，联合用药后坏死细胞比例明显增加。结论顺铂和阿霉素联合作用，可显著地抑制人舌鳞癌细胞分化增殖，并增强药物的细胞毒性作用。
Objective To study the anti-tumor effect of cisplatin and adriamycin combination on the growth, cell cycle and apoptosis of tongue squamous cancer cell line Tca8113. Methods Different concentrations of cisplatin and/or adriamycin were added to Tca8113 cells. Cells proliferation was detected by MTT assay, and cell cycle and apoptosis detected by flow cytometry. Results Cisplatin （r=0.538, P〈0.01） and adriamyein （r=0.642, P〈0.01 ）inhibited cell growth in a dose- and time-dependent manner. Addition of 1.25 μ mol/L adr/amycln alone for 24 hours did not show any inhibition on the growth of TcaSll3. With cisplatin （2-10 rag/L） alone for 24 hours, only 10 mg/L cisplatin inhibited the growth of Tea8113 cells significantly （P〈0.01）. Combined with 1.25 μmol/L adriamycin, 24-hour treatment of cisplastin inhibited the cell growth at the levels of 4, 6, 8 and 10 mg/L which was significantly different as compared to the same level of cisplatin alone （all P〈0.001 ）. 1.25p, mol/L adriamycin, 6 mg/L cisplatin and adriamycin 1.25 μmol/L ＋ cisplatin 6 mg/L were all shown to significantly change G0-G1 phase（50.00±0.32%,43.53±2.026%,50.77± 3.83%） and S phase cell percentage （36.5±1.05%,40.8±2.52%, 30.87±2.65%） （all P〈0.01 ）, but the effect was more evident with combination of cisplatin and adriamycin （P〈0.05）. While no obvious effect on apoptosis was found with these drugs, combined use led to higher percentage of necrotizing cells. Conclusion Cisplatin and adriamycin combination can markedly inhibit the cells line Tca8113 differentiation and proliferation, and enhance cytotoxic effect of these two drugs.
出处
《中华生物医学工程杂志》
CAS
2009年第6期431-435,共5页
Chinese Journal of Biomedical Engineering
基金
广州市医药卫生科技项目（2006-YB-170）
关键词
舌癌
顺铂
阿霉素
生长抑制
细胞周期
Tongue carcinoma
Cisplatin
Adriamycin
Growth inhibition
Cell cycle
