摘要
目的：观察去甲二氢愈创木酸（ＮＤＧＡ）对人恶性胶质瘤细胞系ＳＨＧ－４４细胞增殖和分化的影响。方法：细胞培养、免疫组化、光镜和电镜观察及流式细胞仪检测。结果：①在２００μｍｏｌ／Ｌ浓度范围内，ＮＤＧＡ对ＳＨＧ－４４细胞有增殖抑制和诱导分化作用，且这种作用与浓度呈正相关；②在ＮＤＧＡ处理９６ｈ内，ＮＤＧＡ对ＳＨＧ－４４细胞的增殖抑制作用以处理后２４ｈ最明显；③ＮＤＧＡ处理后，ＳＨＧ－４４细胞增殖受阻于Ｇ１→Ｓ期。结论：ＮＤＧＡ对ＳＨＧ－４４细胞有明显的增殖抑制和诱导分化作用，且这种作用有周期特异性。
Objective: To investigate the effects of nordihydroguaiaretic acid (NDGA) on proliferation and differentiation of human malignant glioma cell line SHG 44. Methods: Cell culture, immunohistochemistry, flow cytometry, light and electron microscopy were employed in this study. Results: ①NDGA with a concentration within 200 μmol/L inhibited the proliferation of SHG 44 cells and this effect was positively correlated to the concentration. ②The most conspicuous inhibition of the proliferation was found at the 24 th h after treatment with 100 μmol/L NDGA for up to 96 h. ③Cell cycle revealed that the cell proliferation was blocked in the phase of G1 to S after treatment with NDGA. Conclusion: NDGA can inhibit proliferation and induce differentiation of human malignant glioma cells and this effect is cycle specific.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1999年第1期9-12,共4页
Journal of Third Military Medical University
基金
国家自然科学基金
关键词
NDGA
胶质瘤
分化
细胞增殖
脑肿瘤
nordihydroguaiaretic acid
glioma
induced differentiation
