摘要
目的 探讨大鼠鼻粘膜P物质受体 (substancePreceptor,SP R)mRNA的表达水平及其与变应性鼻炎的关系。方法 选健康Wistar大鼠 2 0只 ,雌雄不限 ,随机分为实验组和对照组各 10只。实验组用卵清蛋白行腹腔注射基础致敏 ,继之鼻局部激发建立大鼠变应性鼻炎模型。以 β 肌动蛋白 (β actin)作内标准 ,利用逆转录 多聚酶链反应 (reversetranscriptivepolymerasechainreaction ,RT PCR) ,对变应性鼻炎模型组和对照组大鼠鼻粘膜SP RmRNA进行了定量研究。同时利用地高辛标记的cDNA探针对其行Southern杂交鉴定。结果 对照组和实验组大鼠鼻粘膜中均有SP RmRNA表达 ,但实验组大鼠中SP RmRNA表达量较对照组明显增多 (P <0 .0 5 )。结论 变应性鼻炎鼻粘膜SP RmRNA表达量增多 ,提示SP RmRNA表达上调在变应性鼻炎的发生发展中起作用。
Objective To study the expression of substance P receptor messenger RNA (SP-R mRNA) in nasal mucosa in allergic rhinitis (AR) rat model. Methods Twenty Wistar rats free of disease were randomly divided into two groups. AR model rats were established through repeated intraperitoneal shot of ovalbumin (OV) and consequently confirmed by local challenge with OV. SP-R mRNA in nasal mucosa, obtained from two groups, were used to do reverse transcriptive polymerase chain reaction (RT-PCR) and Southern blot. β-actin was used as a standard control through out the whole process. Results The results showed definitely that there were positive expression of SP-R mRNA in normal nasal mucosa. This expressions increased significantly ( P <0.05) during nasal allergy. Conclusion Increased expression SP-R mRNA in nasal mucosa in AR model might play roles in the development of AR.
出处
《中华耳鼻咽喉科杂志》
CSCD
2000年第4期247-250,共4页
Chinese Journal of Otorhinolaryngology
基金
山西省自然科学基金!资助项目 (981 60 8)
关键词
变应性鼻炎
鼻粘膜P物质
MRNA
大鼠
Rhinitis,allergic,nonseasonal
Receptors,neurokinin-1
Molecular biology
Rat
