摘要
目的 应用P物质受体 (substancePreceptor,SPR)的多克隆抗体观察豚鼠中耳急性感染后内耳Corti器SPR阳性细胞的表达。方法 豚鼠一侧耳经鼓膜注射 1× 10 8个 /L金黄色葡萄球菌液 ,制备急性化脓性中耳炎模型 ,3d后灌注固定 ,取耳蜗基底膜铺片 ,行SPR免疫组织化学染色。结果 光镜下发现中耳急性感染后豚鼠耳蜗基底膜表达一种非正常耳蜗组织细胞本身的SPR阳性细胞 ,这些细胞与内、外毛细胞 ,支持细胞 ,血管内皮细胞 ,螺旋神经节细胞等在形态部位上存在明显的差异。阳性细胞呈散在分布 ,主要集中在基底膜的游离缘 ,体积较大 ,约为红细胞的 6～ 12倍 ,每个细胞均有多个突起 ,形成类神经元状。细胞呈SPR强阳性。在阳性细胞的胞质内可见空泡或颗粒状的结构。结论 中耳急性感染可诱发豚鼠Corti器表达非正常基底膜所有的SPR阳性的非特异性反应细胞 。
Objective To investigate the expression of substance P receptor(SPR),SPR positive cells in the Corti′s organ with acute middle ear infection in guinea pigs using the polyclonal antibody of SPR Methods Twelve healthy guinea pigs were employed in the experiment After general anesthesia,by injecting 1×10 8/L staphylococcosis aureus into the middle cavity of right ear with the left ear serving as control,the acute middle ear infection model was established Then three days later,immunohistochemical staining of SPR was performed in the Cochlear base membrane preparation Results Microscopic examination of whole cochlear preparation revealed a number of SPR positive cells expression in the cochlear base membrane,these labeled cells usually did not exist in normal cochlear tissue Obvious difference in morphology and distribution could be identified with inner hair cells,outer hair cells vascular endothelial cell and spiral ganglion neurons Labeled SP receptor positive cells were similar to 'neurons',scattering distributed in the free margin of cochlear base membrane,with larger size and multiple projections which was 6 12 times than the erythrocyte.There were the vesicle and granular substances in the cytoplasm of the labeled cells Conclusions Acute middle ear infection could induce the expression of SP receptor positive nonspecific cells in the Corti′s organ of guinea pigs.These cells did not exist in the normal base membrane and might participate in initiating or inducing the immune response of inner ear.
出处
《中华耳鼻咽喉科杂志》
CSCD
北大核心
2004年第2期93-96,共4页
Chinese Journal of Otorhinolaryngology
基金
国家自然科学基金资助项目 ( 3 0 3 715 2 8)
