摘要
目的:探讨外周血中Th1、Th2细胞及相关细胞因子在变应性鼻炎发病中的变化和作用机制。方法:以卵清蛋白致敏激发制成SD大鼠变应性鼻炎模型,以流式细胞仪检测外周血中Th1和Th2细胞的含量,采用酶联免疫吸附试验(ELISA)检测血清和鼻黏膜组织匀浆中的INF-γ,IL-4的含量。结果:与健康对照组相比,变应性鼻炎模型的Th1数量有下降趋势,但P>0.05,Th2细胞却明显升高,P<0.05。Th1/Th2显著偏离正常比值,P<0.01。血清和组织匀浆中INF-γ下降,P>0.05,IL-4含量明显升高,P<0.01。结论:大鼠变应性鼻炎模型外周血中Th2细胞及相关细胞因子的变化,在其发病过程中可能具有重要的意义。
Objective： To evaluate the role of Th1 ,Th2 cells and correlative cytokines in the pathogenesis of allergic rhinitis. Method： Albumin from chicken egg white was used to induce allergic rhinitis in SD rats. Flow cytometry was used to count the Tht and Th2 cells in peripheral blood. And the content of INF-γ and IL-4 in blood serum or mucosa homogenate was mesured by enzyme-linked immunosorbent assay （ELISA） method. Result：Compared with healthy controls, Th1 was slightly lower and Th2 was significantly higher in the models（ P 〈0. 05）.Th1/Th2 ratio showed a clear deviation from normal balance（ P 〈0.01）. INF-γ deereased （but P〉0.05） and IL-4 clearly increased（ P 〈0.01 ） in blood serum or mucosa homogenate. Conclusion： The changes of Th2 cells and correlative eymkines may play an importam role in the pathogenesis of allergic rhinitis.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2005年第17期794-796,共3页
Journal of Clinical Otorhinolaryngology
