摘要
目的:观察芪蓟肾康颗粒总多糖提取物对大鼠肾小球系膜细胞白介素-6(IL-6)、单核细胞趋化蛋白-1(MCP-1)表达的影响。探讨芪蓟肾康颗粒总多糖提取物防治肾小球硬化的作用机制。方法:应用血清药理学方法,制取芪蓟肾康颗粒总多糖高、低剂量组(生药:30,15 g.kg-1)的含药血清;用血管紧张素Ⅱ(AngⅡ)作为刺激因子,培养大鼠肾小球系膜细胞,使其发生增殖;用免疫酶联吸附法(ELISA法)检测系膜细胞IL-6,MCP-1蛋白表达的情况。结果:芪蓟肾康颗粒总多糖对IL-6有显著抑制作用,与AngⅡ组比较,差异显著(P<0.01);芪蓟肾康颗粒总多糖对MCP-1有显著抑制作用,与AngⅡ组比较,差异显著(P<0.01)。结论:芪蓟肾康颗粒总多糖可能通过抑制IL-6,MCP-1的蛋白表达,抑制炎症反应,从而防治肾小球硬化。
Objective： To probe the therapeutic mechanism of Qiji Shenkang granule total polysaccharide on glomerular sclerosis by observing the effect of Qiji Shenkang granule total polysaccharide on the expression of interleukin-6（IL-6）,monocyte chemoattractant protein-1（MCP-1） of mesangial cells of rats.Method： Serum containing Qiji Shenkang granule total polysaccharide was collected by serum pharmacology method.The cultured rat glomerular mesangial cells were stimualted to proliferate by angiotensin II（Ang II）.The protein expression of MCP-1 and IL-6 was measured by ELISA.Result： Qiji Shenkang granule total polysaccharide had markedly inhibitory effect on IL-6 with significant difference compared with the Ang II group（P ＆lt; 0.01）.Qiji Shenkang granule total polysaccharide had remarkably inhibitory effect on MCP-1 with significant difference compared with the Ang II group（P ＆lt; 0.01）.Conclusion： Qiji Shenkang granule total polysaccharide may inhibit the protein expression of IL-6 and MCP-1 to relieve the inflammatory reaction and thereby to prevent and treat glomerular sclerosis.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第12期239-241,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技重大专项项目(2009ZX09103)
辽宁省博士科研启动基金(20091053)
