摘要
目的:研究黄芪多糖(APS)对肾阳虚型糖尿病大鼠肾脏核因子-κB(NF-κB)、转化生长因子-β1(TGF-β1)过度表达的抑制作用。方法:将纯种SD雄性大鼠随机分为4组:正常对照组、模型组、苯那普利治疗组和APS治疗组。实验第8周时统一处死,以RT-PCR法检测各组大鼠肾组织NF-κB mRNA、TGF-β1mRNA表达,并观察肾脏超微结构变化及肾重/体重,测定大鼠血糖(BS)、血脂及肾功能。结果:与苯那普利治疗组相比,APS能使大鼠肾组织NF-κB mRNA、TGF-β1mRNA表达量减少,肾脏病理改变减轻,还可明显降低肾重/体重、血糖、血脂,改善肾功能。结论:糖尿病大鼠肾组织NF-κB、TGF-β1活性增加,APS能抑制NF-κB mRNA、TGF-β1mRNA的过度表达,从而延缓糖尿病肾病的进展。
Objective. To investigate the effect of astragalus polysaccharides （APS） inhibiting NF-κB （nuclear factor-kappa B） and TGF-β1 （transforming growth factor β1 ） in the renal tissue of diabetic rats with the Yang deficiency of kidney（diagnosed by traditional Chinese medical me thod）. Methods： Male SD rats were divided into four groups., the normal control group, diabetic nephropathy group, benazepril treated group and APS treated group. At the end of the 8th week, the kidneys were taken out from all the rats to detect the mRNA expression of NF-κB and TGF- β1 by RT-PCR （reverse transcript polymerase chain reaction）. The ratio of kidney weight to body weight （KW/BW） was measured, as well as the change of renal tissue structure was observed by electronic microscope. Blood glucose （BG）, blood fat and renal function were also measured. Results.. Comparing with benazepril, APS significantly inhibited the expression of NF-κB mRNA and TGF-β1 mRNA, ameliorated the pathological change of renal, decreased the ratio of kidney weight to body weight, BG and blood fat, and improved renal function. Conclusion： NF-κB and TGF-β1 were activated in the diabetic kidneys. APS can decrease the mRNA expression of NF-κB and TGF-β1 in diabetic kidneys and delay the development of diabetic nephropathy.
出处
《武汉大学学报（医学版）》
CAS
2006年第3期381-384,388,共5页
Medical Journal of Wuhan University
基金
湖北省自然科学基金资助项目(编号:2004ABA160)
关键词
糖尿病肾病
肾阳虚
黄芪多糖
核因子-ΚB
转化生长因子-Β1
Diabetic Nephropathy
Deficiency of Kidney-Yang
Astragalus Polysaccharides
Nuclear Kacter- KappB
Transforming Growth Factor-betal
