摘要
目的:优选大孔树脂纯化泥胡菜总黄酮的工艺条件。方法:采用静态吸附-洗脱试验筛选大孔树脂型号,动态吸附法优化大孔树脂纯化泥胡菜总黄酮工艺参数。结果:优选的纯化工艺为选用D101型树脂,上样液质量浓度1.5 g.L-1,上样速率3 BV.h-1,上样体积4 BV,上样液pH 5,加50%乙醇5 BV以2 BV.h-1流速洗脱,泥胡菜总黄酮纯度达47.8%。结论:D101型大孔树脂对泥胡菜总黄酮具有良好的纯化效果。
Objective： To optimize purification technology conditions of total flavonoids from Hemisteptia lyrata by macroporous resin.Method： Two models of macroporous resins were selected by static adsorption-elution test,purification technology of total flavonoids from H.lyrata with macroporous resin was optimized by dynamic adsorption method.Result： D101 resin was adopted,optimized purification technology was as following： the concentration of sample solution 1.5 g.L-1 with pH 5,adsorption velocity 3 BV.h-1,sample volume 4 BV,then eluted with 5 BV 50% ethanol at the speed of 2 BV.h-1,collected eluate.Under these conditions,yield of total flavonoids was 47.8%.Conclusion： D101 macroporous resin could purify total flavonoids from H.lyrata effectively.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第12期34-36,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
福建省自然科学基金课题(2010J01198)
福建省重点高校服务海西项目(No.5)
关键词
泥胡菜
总黄酮
大孔树脂
纯化
Hemisteptia lyrata
total flavonoids
macroporous resin
purification
