摘要
目的研究高压脉冲电场法对黄花乌头中关附甲素的最佳提取工艺,为工业化生产提供可靠的实验数据和理论依据。方法采用正交试验设计优化高压脉冲电场法快速提取方法,HPLC法测定关附甲素的量。结果提取黄花乌头关附甲素的最佳提取工艺条件为以90%乙醇为提取溶剂,电场强度20 kV/cm,脉冲数为10,料液比1∶14,影响因素由大到小依次为电场强度、料液比、脉冲数;此法提取黄花乌头中的关附甲素提取率达到0.391%,浸膏得率为39.92%。结论本提取工艺可行、经济、省时、回收率高。
Objective To optimize the extracting technology for guanfubase A from Aconitum coreanum by high-voltage pulsed electric field method and to provide the reliable experimental data and theoretical basis for the industrial production.Methods The content of guanfubase A was detected by HPLC in orthogonal test.Results The optimal formulation was as follows： high voltage of 20 kV/cm,impulsion frequency of 10,and solid-to-solvent ratio of 1∶14,with 90% ethanol as extracting solvent,and the influence factor in size was voltage,impulsion frequency,and solid-to-solvent ratio.The guanfubase A content and extract yield were up to 0.391% and 39.92% in A.coreanum,respectively.Conclusion The extracting technology is feasible and economic,which has the advantage of time-saving and high recovery rate.
出处
《中草药》
CAS
CSCD
北大核心
2013年第10期1267-1270,共4页
Chinese Traditional and Herbal Drugs
基金
吉林省自然科学基金资助项目(201115131)
吉林市科技局项目(201222306)
关键词
黄花乌头
关附甲素
正交试验
高压脉冲电场
浸膏得率
Aconitum coreanum（Lévl.） Raipaics
guanfubase A
orthogonal test
high-voltage plused electric field
extract yield
