摘要
目的:采用响应面法优化土鳖虫蛋白质的酶解工艺条件。方法:采用甲醛电位滴定法测定氨基氮的含量。以土鳖虫蛋白水解产物氨基氮的含量为指标,考察土鳖虫酶解的最适酶种(胃蛋白酶、胰蛋白酶和木瓜蛋白酶),并在单因素试验的基础上,采用Box-Benhnken响应面分析法,考察酶解温度、pH值和固液比等3个因素对氨基氮含量的影响,得到描述土鳖虫蛋白水解产物氨基氮含量与操作参数之间的2次响应面模型,确定土鳖虫蛋白质的最优酶解工艺条件。结果:最佳工艺条件为:选择胰蛋白酶为最适酶种,在固液比为1∶7、PH为5.0、酶解温度为60℃的条件下,酶解1 h。经验证试验此条件下氨基氮的得率为1.22%,与理论值(1.25%)基本相符。结论:该工艺成本低,蛋白质水解彻底,获得的氨基氮含量高,工艺稳定,重现性好,可为土鳖虫蛋白水解的工业化生产提供科学依据。
Objective ： To optimize the hydrolysis conditions of Eupolyphaga protein by using response surface methodology. Methods： Formaldehyde potentiometric titration was used to select the optimal enzyme from Pepsin,Trypsin and Papain. Amino nitrogen content was chosen as the indicator of Eupolyphaga protein hydrolyzate. Based on the single - factor test, Box - Benhnken response surface method was established for analysing influence factors of amino nitrogen, including hydrolysis temperature, pH and solid - liquid ratio. The quadratic response surface model between amino nitrogen content and operating parameters was gained and the optimal hydrolysis conditions of Eupolyphaga protein was confirmed. Results ： The optimal hydrolysis conditions were as follows ： the ratio of material to liquid was 1 ： 7, pH was 5.0, hydrolysis tem- perature was set at 60℃ and enzymatictime was lh. The amino nitrogen yeild was 1.22% in this condition, which was consistent with the theoretical value of 1.25 %. Conclusion： The optimal hydrolysis condition has the advantage of low expense, full protein hydrolysis, obtaining high content of amino nitrogen and better stability and reproducibility. Therefore, this paper could provide a scientific basis on the industrial production of Eupolyphaga proteolytic.
出处
《中华中医药学刊》
CAS
2013年第6期1223-1227,共5页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省科技厅省级公益性技术应用研究计划资助项目(2011C22004)
浙江省中医药普通课题计划资助项目(2009CA005)
杭州市科技局科技创新攻关重点资助项目(20051323B46)
关键词
土鳖虫
蛋白水解
响应面分析法
工艺优化
Eupolyphaga sinensis Walker
protein, hydrolysis
response surface methodology
process optimization
