摘要
目的 :比较脓毒症时肝脏与肺脏在细胞因子表达上的差异。方法 :盲肠结扎穿孔 (CL P)造成脓毒症小鼠模型 ,假手术组接受同样手术操作但不行 CL P。用逆转录聚合酶链反应 (RT PCR)的方法检测了脓毒症小鼠肝、肺组织中多种细胞因子〔促炎症细胞因子肿瘤坏死因子α(TNFα)、白介素β(IL 1β)、IL 6 ,抗炎症细胞因子 IL 4〕m RNA的表达。结果 :CL P后 ,肺组织中促炎症细胞因子 TNFα、IL 1β、IL 6的表达明显增高 ,而抗炎症细胞因子 IL 4的表达却无明显改变 ;肝组织中在促炎症细胞因子 TNFα、IL 1β、IL 6表达明显增高的同时 ,抗炎症细胞因子 IL 结论 :脓毒症时肝、肺组织中炎症反应的状况和程度是不同的。其中肝脏在脓毒症早期以促炎症反应占主导地位 ,晚期同时还产生了代偿性的抗炎症反应 ;
Objective:To compare the differences of the cytokine gene expression in the liver and lungs during sepsis.Methods:Male NIH mice were subjected to cecal ligation and puncture(CLP) or sham operation (Sham).Proinflammatory cytokine 〔tumor necrosis factorα (TNFα),interleukin1β(IL1β),IL6〕 and antiinflammatory cytokine(IL4) gene expression in liver and lung tissue were assessed by reverse transcriptionpolymerase chain reaction.Results:Significant increases in TNFα,IL1β and IL6 gene expression were observed at 3 and 12 hours after CLP both in the liver and lungs.But the level of IL4 gene expression was not changed after CLP in the lungs,while that in the liver tissue was increased at 12 hours after CLP.Conclusions:The conditions of inflammatory reponse in the liver and lungs appear to be different during sepsis.At the early stage of sepsis,the degree of proinflammatory response was stronger than that of antiinflammatory response both in the liver and lungs,while at the late stage of sepsis,compensatory antiinflammatory response was found in the liver but not in the lungs.
出处
《中国危重病急救医学》
CAS
CSCD
2000年第10期588-590,共3页
Chinese Critical Care Medicine
基金
国家自然科学基金资助项目!(No.39870796)
关键词
脓毒症
细胞因子
基因表达
MODS
multiple organ dysfunction syndrome
sepsis
cytokine
gene expression
