摘要
目的观察地塞米松对大鼠产后出血致多器官功能障碍综合征(MODS)的肺脏和小肠的氧化应激和炎症因子释放的影响。方法产后24 h内的SD成年雌鼠27只,采用随机数值表法将雌鼠分成3组,每组9只。对照组:仅进行麻醉,股动静脉置管;模型组:股动脉放血致失血性休克60 min+肠系膜上动脉夹闭40 min;地塞米松组:在股动脉放血前10 min,股静脉缓慢注射地塞米松(0.5 mg/100 g)。24 h后颈椎脱臼致死,取大鼠的右侧肺脏,小肠,储存在-80℃冰箱备测。把肺脏和小肠组织进行匀浆后测丙二醛(MDA),一氧化氮(NO),超氧化物歧化酶(SOD),谷胱甘肽(GSH),过氧化氢酶(CAT),肿瘤坏死因子-α(TNF-α)和白细胞介素6(IL-6)含量。数据采用单因素方差分析,进一步两两比较采用LSD法。结果与对照组比较,模型组肺脏和小肠的氧化应激指标[MDA(813.34±84.39)vs(366.84±76.59)nmol/g和(342.06±39.42)vs(171.68±29.97)nmol/g,NO(288.22±25.35)vs(133.09±24.86)μmol/g和(834.10±56.37)vs(331.53±24.97)μmol/g]升高,而SOD[(158.50±31.02)vs(233.83±23.37)U/mg和(227.75±41.87)vs(407.35±55.63)U/mg,GSH(155.25±25.70)vs(268.20±28.35)nmol/g和(283.83±63.57)vs(485.71±85.10)nmol/g,CAT(343.75±81.40)vs(473.67±80.04)U/g和(245.33±40.04)vs(494.69±40.25)U/g]降低(P<0.05)。与模型组比较,地塞米松组肺脏和小肠的氧化应激指标[MDA(526.35±56.94)vs(813.34±84.39)和(261.78±56.01)vs(342.06±39.42)nmol/g,NO(192.16±36.37)vs(288.22±25.35)和(594.97±59.42)vs(834.10±56.37)μmol/g]降低,而SOD(201.35±21.29)vs(158.50±31.02)U/mg和(344.10±54.80)vs(227.75±41.87)U/mg,GSH(233.05±32.72)vs(155.25±25.70)nmol/g和(436.72±65.49)vs(283.83±63.57)nmol/g,CAT(406.96±45.40)vs(343.75±81.40)U/g和(370.36±63.56)vs(245.33±40.04)U/g则升高(P<0.05)。与对照组比较,模型组肺脏和小肠的炎症因子TNF-α(853.80±154.17)vs(318.16±66.95)pg/mg和(418.42±76.22)vs(215.89±47.78)pg/mg,IL-6(1673.16±183.98)vs(1138.01±125.50)pg/mg和(765.83±74.05)vs(149.55±35.54)pg/mg显著升高(P<0.05)。与模型组比较,地塞米松组肺脏和小肠的炎症因子TNF-α(652.45±121.36)vs(853.80±154.17)pg/mg和(290.58±35.54)vs(418.42±76.22)pg/mg,IL-6(1373.24±114.87)vs(1673.16±183.98)pg/mg和(523.91±68.81)vs(765.83±74.05)pg/mg显著降低(P<0.05)。结论地塞米松可以减少大鼠产后出血致多器官功能障碍综合征的肺脏和小肠氧化应激损伤,同时抑制肺脏和小肠组织的炎症因子TNF-α和IL-6的释放。
Objective To evaluate the effect of dexamethasone on oxidative stress and inflammatory factor release in postpartum hemorrhage and multiple organ dysfunction syndrome rats. Methods Twenty-seven postpartum Sprague-Dawley rats were randomly divided into 3 groups, which were model group, dexamethasone group and control group. Each group included 9 rats. The rats in control group only received vein indwelling needle in femoral artery and femoral vein after general anesthesia. The rats in model group exposed to hypotension for 60 minutes and superior mesenterie artery being clamped for 40 minutes after all the procedure in control group. While the rats in dexamethasone group received dexamethasone （0. 5 rag/100 g） before the procedure in the model group. All the rats were euthanized after 24 hours at the end of experiment. The right lung and small intestine were stored at minus 80 degrees centigrade. Malondialdehyde （ MDA ）, nitric oxide （ NO ） , superoxide dismutase （ SOD ）, glutathione （ GSH）, catalase （ CAT）, tumor necrosis factor-α （TNF-α） and interleukin- 6 （ IL- 6 ） were assayed. The data were analyzed using one-way ANOVA, and further analysis was using LSD test. Results Oxidative stress markers MDA（813.34 ±84. 39 and 342. 06±39.42） ,NO （288.22 ±25.35 and 834. 10 ±56. 37） levels increased in model group compared with control group rats （366. 84 ± 76. 59 and 171.68 ± 29.97, 133.09±24. 86 and 331.53 ±4. 97）, all P 〈0. 05. The levels of oxidative stress markers SOD （ 158. 50 ±31.02 and 227.75 ±41.87 ）, GSH （155.25 ±25.70 and 283.83 ± 63.57 ） and CAT（ 343.75 ± 81.40 and 245.33 ± 40. 04） in model group were significant decreased compared with control group rats （233.83 ±23.37 and 407.35 ±5.63, 268.20 ±8.35 and 485.71 ±5.10, 473.67 ± 80. 04 and 494. 69 ±40. 25）, all P 〈 0. 05. The levels of oxidative stress markers MDA （526. 35 ±56. 94 and 261.78 ±56. 01 ）, NO（ 192. 16 ±36. 37 and 594. 97 ±59.42） in dexamethasone group were lower than those in model group（813. 34 -±84. 39 and 342. 06 ±9. 42, 288. 22 -±25.35 and 834. 10 ±6. 37）, all P 〈0. 05. The levels of oxidative stress markers SOD （ 201.35 -± 21.29 and 344. 10 ± 54. 80）, GSH （ 233.05 ±32. 72 and 436. 72 -± 65.49 ） and CAT （ 406. 96 ±45.40 and 370. 36 ±63.56 ） in dexamethasone group were higher than model group （ 158. 50 ±31.02 and 227.75 ± 41.87, 155.25 ±25.70 and 283.83 ±63.57, 343.75 ± 81.40 and 245.33 ±40.04）, all P 〈 0. 05. Inflammatory factor TNF- α（ 853.80 ±154. 17 and 418.42 ± 76. 22） , IL-6 （ 1673.16 ±183.98 and 765.83 ±74. 05 ） increased in model group compared with control group rats （318. 16 ±66.95 and 215.89 ±7.78, 1138.01 ± 125.50 and 149.55 ±35.54）, all P 〈 0.05. Inflammatory factor TNF-cL （652.45± 121.36 and 290.58 ±35.54）, IL-6 （1373.24 ± 114. 87 and 523.91 ± 68.81 ） in dexamethasone group were lower than those in model group （853.80± 154. 17 and 418.42 ±6.22, 1673.16 ± 183.98 and 765.83 ±4.05）, all P 〈0.05. Coneluslon Dexamethasone may decrease oxidative stress injury and inhibit inflammatory factors TNF-α and IL-6 release in the postpartum hemorrhage and multiple organs dysfunction syndrome rats.
出处
《中华产科急救电子杂志》
2013年第3期47-51,共5页
Chinese Journal of Obstetric Emergency（Electronic Edition）
基金
广东省科技厅项目(2012B020700007)
广州市科技和信息化局项目(2013J4100039)
关键词
地塞米松
产后出血
多器官功能衰竭
Dexamethasone
Postpartum hemorrhage
Multiple organ failure
Rats,Sprague-Dawley
