摘要
目的:优选丹参活血化瘀有效组分的最佳提取纯化工艺。方法:以隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA,作为丹参活血化瘀作用脂溶性有效组分的指标成分,丹酚酸B为水溶性有效组分的指标成分,考察不同的提取、纯化方式,并优选出提取纯化的最佳工艺条件。结果:丹参中脂溶性的总丹参酮有效组分采用乙醇渗漉法提取,最佳工艺为6倍量的乙醇以5mL·min-1·Kg-1的速度渗漉提取;水沉法纯化,最佳条件为20倍生药材量的水进行沉淀。丹参中水溶性的总酚酸有效组分采用水提法,对丹参渗漉醇提后的药渣进行水提取,最佳工艺为8倍量水提取2次每次1小时;大孔树脂纯化,最佳条件为AB-8型大孔吸附树脂,0.5g生药/mL样品以1mL·min-1的流速吸附,最大上样体积为60mL,再以10倍药材量,流速1mL·min-1的30%乙醇洗脱。结论:优选最佳工艺得到的丹参活血化瘀有效部位中总丹参酮和总酚酸含量均较高,且工艺简单,适宜工业化生产。
Objective To optimize a technique for extracting and purifying blood-activating and stasis-removing components from Sal- via miltiorrhiza. Methods Cryptotanshinone, tanshinone I , tanshinone I1 A were selected as marker components for liposoluble part, while salvianolic acid B for water-soluble part of Salvia miltiorrhiza. Various extraction, purification methods were investigated, further- more the best extraction and purification process optimized. Results For liposoluble total tanshinones, ethanol percolation was adopted as extraction method with the best process parameters of 6 times volume ethanol, 5ml ~ rain＇~ ~ Kg-~ speed; and water precipitation was se- lected as purification method with the best water amount of 20 times. For water-soluble total phenolic acid, water extraction method was adopted with 8 times volume, twice, 1 hour per extraction; and macroporous resin was selected as purification method with the best pro- cess as follows： AB-8 models, 0.5g/mL crude drug, 1 mL ~ min-~ absorption speed, maximum sample volume of 60 mL, 10 times and 30% ethanol elution with 1 mL ~ min-~ speed.Conclusion The content of the total tanshinones and phenolic acid obtained were both high by the optimum extraction-purification conditions.The technique is simole and suitable for the modern oroduction.
出处
《江西中医学院学报》
2012年第5期52-55,共4页
Journal of Jiangxi College of Traditional Chinese Medicine
基金
国家科技支撑计划项目(项目编号:2008BAI51B03)
江西中医学院重点学科青年教师培养计划资助项目(项目编号:2012jzzdxk008)
关键词
丹参
丹参酮类
酚酸类
提取
纯化
Salvia Miltiorrhiza
Tanshinones
Phenolic Acid
Extraction
Purification
