摘要
目的:优选妇舒保凝胶中黄柏、苦参的提取工艺。方法:以出固率和盐酸小檗碱、苦参碱的得率为指标,单因素试验考察水煎煮法、乙醇回流法及乙醇渗漉法对黄柏、苦参提取效率的影响;选取乙醇体积分数、乙醇用量、提取次数、提取时间为考察因素,采用正交试验优选妇舒保凝胶中黄柏、苦参的提取工艺。结果:选择乙醇回流法进行提取,最佳提取工艺为加8倍量50%乙醇提取3次,每次1.5 h,总生物碱得率约2.47%。结论:该优选工艺稳定可行、重复性良好,为妇舒保凝胶中有效成分的提取提供试验依据。
Objective： To optimize extraction technology of PheUodendrom chinense and Sophora flavescens from Fushubao gel. Method： Taking yield of matrine, berberine hydrochloride and extract as indexes, single factor test was used to choose extraction method, including water boiling method, ethanol reflux method and ethanol percolation method; Orthogonal test was designed to optimize extraction technology of P. chinense and S. flavescens from Fushubao gel with extraction times, extraction time, the amount and concentration of ethanol as factors. Result： Ethanol reflux method was selected, optimum extraction technology was as follows： extracted 3 times with 8 times the amount of 50% ethanol, 1.5 hours each time. Yield of total alkaloid was about 2.47%. Conclusion： This optimized process was stable, feasible and reproducible, it could provide experimental basis for extraction of active ingredients from Fushubao gel.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第2期46-48,共3页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
妇舒保凝胶
提取方法
正交试验
黄柏
苦参
Fushubao gel
extraction technology
orthogonal experiment
Phellodendrom chinense
Sophorajavescens
