摘要
利用薄层色谱(TLC)技术对中药材丹参的有效成分进行了指纹图谱分析.以不同等级的中江丹参和不同采收期的丹参、南丹参为分析对象,采用适宜的水溶性及脂溶性成分提取方法和TLC分析条件,可获得其相应的TLC图像及色谱图.通过对不同丹参药材的色谱图像的分析,结合化学计量学方法对其各主要指标成分的信息进行提取,初步构建了丹参的TLC指纹图谱.结果表明,在254 nm波长下获取脂溶性和水溶性成分共7个共有指纹峰,各峰相对比移值(Rf)和峰面积的相对标准偏差分别小于1.7%和4.0%.通过对薄层色谱斑点的Rf值、斑点颜色和大小的比较,并进行主成分分析和聚类分析,实现了对不同等级的丹参进行归属,并确立了最佳采收期.本实验建立的薄层色谱指纹图谱方法具有较高的稳定性、良好的精密度和重现性,可用于丹参药材的质量评价和控制.
The purpose of this study is to develop and apply the thin layer chromatography （TLC） technique to the fingerprinting studies of Danshen （Radix Salvia Miltiorrhiza） drugs. A range of Danshen samples with different commercial grades or harvested after different growing time was analyzed for quality assessment and regulatory purposes. And the compositional variations of these authentic Danshen drugs were investigated using an analytical protocol involving water or organic solvent extractions,followed by TLC assay of the extracts,and subsequent TLC fingerprinting analysis by image comparison combined with chemometrics methods. The analytical performances of the method were evaluated by examining the stability, precision and reproducibility,respectively. Excellent results were observed and the RSDs of relative Rf values and peak areas were respectively less than 1.7 % and 4.0 %. The analysis results obtained suggested that a total of seven common peaks were identified in the TLC chromatograms under the 254 nm detection wavelength. Based on the comparison of the Rf values of the common peaks,the depth and size of the TLC spots and in combination with cluster analysis and principal component analysis （PCA） ,the attribute of quality grading and optimal harvest time of the Danshen material were identified. Thus,the developed TLC chromatographic fingerprinting method is considered as an useful and effective tool for the quality control of the Danshen drug material.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第6期801-805,共5页
Journal of Xiamen University：Natural Science
基金
科技部"九五"攻关计划中药现代化重中之重项目:中药现代化生产中重金属测定的标准化及质量控制(99-929-02-13)
香港创新科技署与香港新世界生物科技有限公司联合资助项目
关键词
丹参药材
TLC指纹图谱
图像分析
化学计量学
质量评价
Radix Salvia Miltiorrhiza
TLC fingerprinting
image analysis
chemometrics
quality assessment
