摘要
The optimal conditions of the conversion of 16α methyl 3β,17α,21 trihydroxy 5α pregnane 20 one 21 acetate to 16α methyl 11α,17α,21 trihydroxy 1,4 pregndiene 3,20 dione by the mixed cultures of Arthrobacter sp 86 and Metarhizium sp 88 were studied.2%～4%( V/V )N,N dimethylformamide in the medium was used to dissolve the substrate.It could increase the conversion ability.Adding the mycelium of Metarhizium into the dehydrogenation reaction mixture during 0～28 hours had no influence on the conversion rate.The optimal concentration of mycelium was around 75 mg/mL wet weight.0.04%～0.06% CoCl 2·6H 2O inhibited the degradation of steroid nucleus so that the product was greatly accumulated.Under such condition the yeild of mixed microbial conversion was 67%.
The optimal conditions of the conversion of 16α methyl 3β,17α,21 trihydroxy 5α pregnane 20 one 21 acetate to 16α methyl 11α,17α,21 trihydroxy 1,4 pregndiene 3,20 dione by the mixed cultures of Arthrobacter sp 86 and Metarhizium sp 88 were studied.2%～4%( V/V )N,N dimethylformamide in the medium was used to dissolve the substrate.It could increase the conversion ability.Adding the mycelium of Metarhizium into the dehydrogenation reaction mixture during 0～28 hours had no influence on the conversion rate.The optimal concentration of mycelium was around 75 mg/mL wet weight.0.04%～0.06% CoCl 2·6H 2O inhibited the degradation of steroid nucleus so that the product was greatly accumulated.Under such condition the yeild of mixed microbial conversion was 67%.
出处
《生物工程学报》
CAS
CSCD
北大核心
2000年第6期763-765,共3页
Chinese Journal of Biotechnology
基金
国家科技攻关资助项目!( 75 71 0 7 0 2 )
关键词
微生物混合转化
地塞美松
中间体
脱氢
羟基化
Arthrobacter,Metarhizium, mixed microbial conversion,16α methyl 11α,17α,21 trihydroxy 1,4 pregndiene 3,20 dione
