摘要
Two kinds of micro organism, Arthrobacter sp. AX86(1,4 dehdrogenator)and Absidia sp. A28(11α hydroxylator)were used in this experiment.Two different fermentation techniques were performed to accomplish the multiple conversional reactions for producing 16β methyl 11α,17α,21 trihydroxy 1,4 pregnadiene 3,20 dione(Ⅲ)from 16β methyl 3β,17α,21 trihydroxy 5α pregnane 20 one 21 acetate(I):1)To produce product(Ⅲ)by means of a two step fermentation method which were independently performed first by Arthrobacter and next by Absiaia, and 2)the product was obtained by a sequential fermentation system of aforesaid two micro organisms in a single fermentor without isolation of the intermediates from the mixture.Our results showed that in both fermentation systems high yield of product was obtained.However,according to the technical simplicity,shorter duration of fermentation cycle and efficient yield of product,the second method is better than the first one.
Two kinds of micro organism, Arthrobacter sp. AX86(1,4 dehdrogenator)and Absidia sp. A28(11α hydroxylator)were used in this experiment.Two different fermentation techniques were performed to accomplish the multiple conversional reactions for producing 16β methyl 11α,17α,21 trihydroxy 1,4 pregnadiene 3,20 dione(Ⅲ)from 16β methyl 3β,17α,21 trihydroxy 5α pregnane 20 one 21 acetate(I):1)To produce product(Ⅲ)by means of a two step fermentation method which were independently performed first by Arthrobacter and next by Absiaia, and 2)the product was obtained by a sequential fermentation system of aforesaid two micro organisms in a single fermentor without isolation of the intermediates from the mixture.Our results showed that in both fermentation systems high yield of product was obtained.However,according to the technical simplicity,shorter duration of fermentation cycle and efficient yield of product,the second method is better than the first one.
出处
《生物工程学报》
CAS
CSCD
北大核心
2000年第5期651-653,共3页
Chinese Journal of Biotechnology
关键词
微生物转化
甾体化合物
脱氢
羟基化
Arthrobacter,Absidia, multiple microbial conversion 16β methyl 11α,17α,21 trihydroxy 1,4 pregnadiene 3,20 dione
