摘要
对白首乌C21甾苷元告达庭甾苷元和开德甾苷元（Ⅱ）实现C11α-羟基化，要求副产物少，收率高。采用赭曲霉（Aspergillus ochraceus）和犁头霉（Absidia sp．）两种微生物在双水相体系中同步转化的方法，羟化反应中控制pH值在6．0～5．0，于30℃温度下振荡培养70h；结果，产物经IR、MS、NMR分析确认是C11α-羟基化了的告达庭甾苷元（Ⅲ）和开德甾苷元（Ⅲ），副产物少，收率达70．1％。赭曲霉（Aspergillus ochraceus）和犁头霉（Absidia sp．）两种微生物在双水相体系中同步转化的方法是实现C11α-羟基化的有效方法。
Two types of Cynanchum auriculatum C21 steroidal aglycones （ Ⅱ ）, such as Caudatin steroidal aglycones and Kaider steroidal aglycones, were C11α-hydroxylated, with a little byproduct and high productivity. C21 steroidal aglycones, namely, Caudatin steroidal aglycones and Kaider steroidal aglycones （Ⅲ） were prepared by biotransformation with Aspergillus ochraceus and Absidia sp. In aqueous two- phase system at pH 6.0-5.0, 30℃ and 70 h. The results showed that the products were identified as two of C11α- hydroxylated cynanchum auriculatum Caudatin steroidal aglycones and Kaider steroidal aglycones（Ⅲ） by IR, MS, and NMR. Two microorganisms （Aspergillus ochraceus and Absidia sp. ） enhanced the C11α- hydroxylation reaction of C21 steroidal aglycones simulteously in aqueous two-phase system effeetively.
出处
《药物生物技术》
CAS
CSCD
2009年第3期245-249,共5页
Pharmaceutical Biotechnology
基金
湖北省教育厅项目
基金编号:2004D003
关键词
赭曲霉
犁头霉
C11α-羟基化
同步转化
双水相系统
Aspergillus ochraceus, Absidia, mul, C11α- hydroxylation, Synchronous transformation, Aqueous two-phase system
