摘要
采用水平淀粉凝胶电泳技术对采集于黄海海州湾海域的真鲷野生群体和经过 2代人工繁育的养殖群体进行了同工酶遗传变异研究。分析检测了 2个群体各 50个样本肌肉和肝脏组织的 1 3种同工酶共 2 0个基因位点 ,其中MDH_a、GPI、PGM等 1 0个位点为多态位点 ,ME和EST_b为变异程度比较高的位点。野生群体和人工繁殖群体的多态位点比例分别为 45%和 2 5% (P0 .95) ;群体平均观察杂合度分别为 0 .1 41± 0 .0 4 4和 0 .0 95± 0 .0 4 3。结果表明 ,真鲷的野生群体和养殖群体拥有较高程度的遗传变异水平 ,但是养殖群体的遗传变异水平比野生群体有一定程度的降低。养殖群体遗传变异水平的降低在一定程度上是由于亲鱼数量少所致。比较了同工酶分析和RAPD分析的结果。将此两种技术相结合在鱼类群体遗传多样性分析中具有比较实际的意义。
To reveal isozyme variation of Red Seabream ( Pagrus major ), each 50 index samples of a wild population and of a hatchery population from Haizhou Bay of Yellow Sea were investigated through starch gel electrophoresis. Twenty loci of 13 enzymes were detected. The proportion of polymorphic loci was 45% and 25% ( P 0.95 ), respectively, ME and EST_b showed the highest genetic variation among the detected loci. The mean heterozygosity ( Ho ) in two populations were 0.141±0.044 and 0.095±0.043, respectively. The hatchery population possesses lower genetic variation than the wild populations. The relatively small numbers of broodstock may result in the lower genetic variation of hatchery population. For the sustainable development of the mariculture in the future, some appropriate measures, such as enlarging the broodstock number, should be taken in case of the reducing of population genetic variation. The result of isozyme analyzing was compared with that of RAPD technique in this passage, which showed that these two results were identical. So it is meaningful to combine RAPD with isozyme technique in assessing population genetic variation.
出处
《生物多样性》
CAS
CSCD
2000年第4期391-396,共6页
Biodiversity Science
基金
国家自然科学基金资助项目!(编号 39630 2 60 )
关键词
真鲷
同工酶
遗传变异
水平淀粉凝胶电泳
red seabream ( Pagrus major ), isozyme, genetic variation, horizontal starch gel electrophoresis
