摘要
本实验采用卡介苗注射器将羊生长激素基因溶液注射到亲虾纳精囊中，以精子为载体，携带基因导入对虾受精卵，而达到转基因对虾的目的。经ＰＣＲ检测表明：１．经ＳＴＥ（ｐＨ８．０）第一次洗涤精子的洗涤液呈阳性信号；第２－４次的洗涤液呈阴性；２．多次洗涤后的精子抽提的ＤＮＡ呈阳性；３．检测 １０个样品１００尾仔虾中有一个样品呈阳性结果，转基因比率在 １％以上。同时斑点杂交结果也印证了上述结果。
The sheep growth hormone gene solution is injected directly into the thelycum of shrimp, Penaeus chinensis by bdcillus Calnlette- Guerin syringe, and the sperm is tested to be used as exogenous DNA vector. PCR results are as follows: 1. The STE lotion of the first time is positive, but the STE lotions of the second to forth time are negative respectively. 2. DNA extracted from washed sperms is positive. 3. About 100 postlarvae are divided into 10 groups and one of those 10 groups is positive. The gene transfer ratio, examined to the test results, is more than or at least 1%. The results mentioned above are also proved by dot blot.
出处
《中国水产科学》
CAS
CSCD
1996年第1期6-10,共5页
Journal of Fishery Sciences of China
关键词
中国对虾
精子
载体
基因导入
Penaeus chinensis, Sperm, Vector, Gene introduction
