摘要
以山葡萄籽为实验材料,通过单因素法提取葡萄籽中的多酚。根据不同浸提剂在540nm处可见光吸收情况的测定,确定70%甲醇溶液为最佳浸提剂。通过单因素试验确定了提取剂浓度、提取时间、提取温度、料液比的适用范围,再利用正交试验法进行工艺优化。确定最佳工艺条件为:体积分数70%的甲醇,料液比1∶20(m/v),70℃水浴,浸提20min。山葡萄籽中多酚的含量可达18.981mg/g。
This paper examined the polyphenols extracted from grape seed according to the different extraction agent in the visible absorption of 540nm place, Determine the best solution for 70% of methanol extraction agent. By single factor experiment, determine the extraction agent concentration, temperature, time, ratio of the applicable scope, Reuse orthogonal test method for optimizing process. Determine the optimum technological conditions for： Volume fraction 70% of methanol, more than 1 ： 20 （m/v）, 70℃ extraction 20 min baths. The seeds of vitis amurensis rupr polyphenol content can reach 18. 981 mg/g.
出处
《中国林副特产》
2013年第2期10-12,共3页
Forest By-product and Speciality in China
基金
鞍山师范学院2011年校级教师科研项目11kyxm44资助项目
关键词
葡萄籽
多酚
正交试验
Grape seed
Polyphenols
The orthogonal experiment
