摘要
将汉滩病毒S片段编码区基因插入到含CMV启动子 /增强子 (promoter/enhancer)的真核表达载体pVR10 12中 ,构建成真核表达质粒 pVRS2 2。质粒DNA经纯化后 ,注射经布比卡因预处理的Balb/c小鼠的股四头肌 ,多次免疫后 ,免疫小鼠淋巴细胞增殖功能的检测结果显示 :免疫鼠的脾细胞能够对体外抗原刺激产生增殖反应 ;CTL活性检测结果表明 :靶细胞51Cr的释放是效应细胞依赖性的 ,并且与病毒感染组的淋巴细胞的细胞毒活性相似。结果显示 ,用重组质粒pVRS2 2免疫小鼠 ,能够诱导小鼠产生特异性淋巴细胞增殖反应和细胞毒性T淋巴细胞活性(CTL)。
Hantaan virus S segment coding region gene was inserted into the eukaryotic expression vector pVR1012 under the control of cytomegalovirus (CMV) promoter/enhancer. The recombinant plasmid DNA, designated as pVRS22 were purified with PEG. Then Balb/c mice were immunized with purified pVRS22 by intramuscular injection at intervals of three weeks for 9 weeks. The lymphoproliferative responses of spleen cells from pVRS22 plasmid injected mice clearly showed that these cells were able to proliferate in the presence of Hantaan virus. The CTL activities of spleen cells from pVRS22 DNA-vaccinated mice were tested with different effector to target ratios. The results demonstrated that the release of chromium from target cells was an effector cell depended phenomenon and were similar to the CTL activities of mice infected with Hantaan virus. Our results showed that specific cell mediated immunity responses were elicited in the pVRS22 vaccinated mice. The lymphoproliferative responses and CTL activity of spleen cells from pVRS22 plasmid vaccinated mice were significant and successful.
出处
《病毒学报》
CAS
CSCD
北大核心
2000年第3期219-222,共4页
Chinese Journal of Virology
关键词
汉滩病毒
基因免疫
S片段编码区基因
Hantaan virus
gene immunization
S segment coding region gene
cytotoxicT lymphocyte (CTL)
recombinant plasmid
