摘要
目的 研究大肠杆菌表达的汉滩病毒囊膜糖蛋白G1和 G2的免疫原性和免疫保护作用 .方法 将大肠杆菌表达的 G1和 G2免疫小白鼠 ,测定免疫小鼠血清中汉滩病毒抗体的间接免疫荧光抗体和中和抗体滴度并利用被动免疫保护试验观察 G1和 G2免疫小鼠血清和脾细跑对汉滩病毒致死性感染乳鼠的保护作用 .结果 表达 G1和 G2免疫小鼠血清中抗汉滩病毒抗体的滴度分别为 1∶ 16 0 ,1∶ 32 0 ;G1,G2共同免疫不能提高免疫效果 ;NP,G1,G2共同免疫 ,血清中抗汉滩病毒抗体的滴度较 NP单独免疫低 ;有一份 G2免疫血清中检测到低效价的中和抗体 ,效价为 1∶ 10 ;G1,G2免疫血清及免疫小鼠脾细胞对致死性感染的乳鼠都有一定保护作用 ,且 G2较明显 .结论 表达的 G2能刺激小白鼠产生中和抗体 ,但表达的 G1不能刺激产生可测定的中和抗体 ,表达的G1,G2和 NP共同免疫不能提高中和抗体的滴度 ;G1和 G2免疫血清和脾细胞都能被动保护致死性感染的乳鼠 ,或使乳鼠免于死亡或延长其存活时间 ,G2的保护作用更明显 ,G2免疫血清的保护率可达 5 0 % ,脾细胞的保护率可达 10 % .
AIM To study the immunoprotection of Hantaan virus envelope glycoproteins G1 and G2 expressed in E.coli . METHODS Mice were immunized with expression G1 andG2, the IFA and neutralizing antibody were tested, and the passive immunoprotection of sera and spleen cells of immunized mice was observed by transfering the sera and spleen cells to fatal Hantaan virus infectious infant mice. RESULTS Antibodies against Hantaan virus could be tested in the sera of the mice immunized with expression G1 and G2, and the IFA antibody titers were 1∶160 and 1∶320 respectively. Expression G2 could stimulate mice to produce neutralizing antibody, the titer being 1∶10, but the neutralizing antibody could not be tested in sera of mice immunized with expression G1. CONCLUSION These findings suggest that the sera and spleen cells of mice immunized with expression G1 and G2 could protect infant mice from death or prolong their life time. It was better in expression G2 than in G1. The maximal protection rates of spleen cells and sera of G2 were 50% and 10% respectively.
出处
《第四军医大学学报》
2000年第7期861-864,共4页
Journal of the Fourth Military Medical University
关键词
汉坦病毒
囊膜糖蛋白
免疫原性
免疫保护
Hantaan virus
recombinant envelope glycoproteins
immunoprotection
