摘要
采用简单且方便的超声方法合成了具有叶酸受体(FR)靶向性的γ-环糊精-叶酸包合物(γ-CD/FA)修饰的CdSe/ZnS量子点(QDs)纳米粒。利用傅里叶红外光谱(FTIR)、核磁共振氢谱(1H NMR)、紫外可见吸收光谱(UV-vis)、荧光光谱和透射电子显微镜(TEM)等手段对其进行了表征。结果表明,FR靶向性的γ-CD/FA修饰的CdSe/ZnS QDs,不但具有较好的单分散性和较小的尺寸,还具有较高的量子产率(QY)和较长的荧光寿命等较好的光学性能。细胞毒性实验表明,FR靶向性的γ-CD/FA修饰的CdSe/ZnS QDs具有较低的毒性,还具有选择性地进入FR高表达的癌细胞内的功能。这种直径为4～5 nm的QDs比较容易进入细胞,在体内易被肾脏清除,比较适合于在生物探针或生物成像等生物医学方面的应用。
The y-cyclodextrin-folate （y-CD/FA） inclusion-coated CdSe/ZnS quantum dots （QDs） with folate- receptor （FR） targeted were synthesized by simple and convenient sonochemical method. The products were studied using fourier transform infrared （FTIR）, proton nuclear magnetic resonance （ 1H NMR）, utraviolet-visible spectrometry （UV-vis）, fluorescence spectrum and transmission electron micrographs （TEM）. The results showed that the y-CD/FA-coated CdSe/ZnS QDs not only have good monodispersity and smaller size, but also have good optical performance, such as higher quantum yield （QY） and a long fluorescence lifetime. The cytotoxicity experiments showed that the y-CD/FA-coated CdSe/ZnS QDs have lower cytotoxicity and could more effectively enter cancer cells with FR over-expression. The QDs with 4-5 nm in diameter were relatively easy to enter the cell and to be removed through kidneys, so it is more suitable for biomedical applications for bioprobes and bioimaging.
出处
《药学学报》
CAS
CSCD
北大核心
2013年第4期566-572,共7页
Acta Pharmaceutica Sinica
基金
国家自然科学基金-河南人才培养联合基金(U1204201)
中国博士后科学基金面上资助(2012M511570)
关键词
量子点
叶酸
环糊精
肿瘤细胞
光学成像
quantum dot
folic acid
cyclodextrin
tumor cell line
optical imaging
