摘要
目的 探讨创伤性脑水肿的发生机制及前列环素的衍生物Iloprost对创伤性脑水肿的治疗作用。方法干湿重法测定脑组织含水量，甲酞胺法测定伊文思蓝(EB）含量，Fura－2／AM荧光标记法测定突触体内［Ca^(2+)］i，并采用前列腺环素稳定的衍牛物Iloprost进行治疗，研究其对EB含量、[Ca^(2+)］i及脑水肿的影响。结果 创伤后2 h即已发生钙超载，脑组织水含量及EB含量的增加，Iloprost治疗后EB含量和[Ca^(2+)］i明显下降，脑水肿明显减轻。结论 血脑屏障的通透性增加和细胞内钙通道开放、钙离子超载在创伤性脑水肿的发生发展中起重要作用，Iloprost对创伤性脑水肿有治疗和脑保护作用。
Objective The authors reported the results of the experiment which was carried out in Wistar rats to observe the mechanisms of traumatic brain edema and to evaluate the therapeutic effect of Iloprost on it. Methods The Evens blue(EB) and water contents of brain tissues and the [Ca2+]i level within the neuronal synapses were determined pre-and post-traumatic brain injury in the rats. These data were then compared with those obtained after administration of Iloprast ( a stable derivative of prostacy-clin). Results Overload of [Ca2+]i in the neuronal synapses was observed as early as 2h following brain injury. Both the water and EB contents in the brain tissue were also significantly increased as compared with that in the control group. When Iloprast was administrated, the EB content and [Ca2+]i dropped dramatically and brainedema was significantly relieved. Conclusions Increase in the permeability of blood brain barrier and [Ca2+]i probably plays a role in the pathogenesis of brain edema after the injury. The authors suggested that Iloprast might have preventive as well therapeutic effects on the patents with traumatic brain injury.
出处
《中国临床神经外科杂志》
2000年第2期97-99,共3页
Chinese Journal of Clinical Neurosurgery
关键词
脑水肿
ILOPROST
创伤
脑损伤
Brain edema Iloprost Wounds and injuries Brain injuries, laboratory
