摘要
目的探讨支气管哮喘（哮喘）患儿IL-10启动子区-592C／A多态性及其对IL．10水平的影响。方法应用病例对照研究方法，选择哮喘患儿及健康儿童各92例。采用PCR．限制性片段长度多态（RFLP）法检测IL-10基因-592C／A多态性，分别计算2组基因型和等位基因频率，组间比较采用，检验。采用ELISA法测定哮喘组患儿发作期、缓解期及健康儿童血清IL-10水平，进行比较；同时比较不同基因型个体血清IL-10水平的差异。组间比较采用F检验以及q检验。结果与健康对照组比较，哮喘组患儿IL-10基因-592C／A多态性分布具有明显差异，哮喘组AA基因型频率（56．5％）和A等位基因频率（73．9％）均较健康对照组（34．8％、58．7％）高，差异均有统计学意义（X2=9．32，P〈0．01;X2=8．87，P〈0．005）。携带AA基因型和A等位基因的儿童与cc基因型和C等位基因个体相比发生哮喘的相对风险分别增加3．25倍（95％CI：1．28-8．28）和1．99倍（95％CI：1．28-3．08）。哮喘患儿血清IL-10水平在发作期以及缓解期均较健康对照组儿童低，差异均有统计学意义（P均〈0．01）；AA基因型个体较CC基因型个体IL-10表达水平低，差异有统计学意义（P〈0．05）。结论哮喘儿童IL-10基因-592C／A多态性与健康儿童存在明显差异，这种多态性影响血清IL-10水平，AA基因型个体IL-10表达水平低，A等位基因可能是儿童哮喘的易感基因。
Objective To explore the polymorphism of - 592C/A of IL-10 gene promoter region in children with bronchial asthma and its relationship with serum concentration of IL-10. Methods Ninety-two children with bronchial asthma and 92 healthy children were selected for study, polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） were used for the analysis of -592C/A of IL-10 promoter region polymorphism. The frequencies of genotypes of IL-10 gene -592 locus （CC, CA and AA） and alleles were accountedrespectively, andX2 test was used to analyze the difference between the groups. Enzyme-linked immunosorbent assay （ELISA） was used to measure the concentration of the serum IL-10,and F test and q test were used for statistical analysis, Results Compared with the healthy control group, there were significant differences in -592C/A polymorphism of IL-10 gene in asthma group. The frequencies of AA genotypes （56.5%） and A allele （73.9%）in asthma group were higher than those （34.8%, 58.7% ） in the control group ,there were significant differences （X2 = 9.32 ,P 〈 0.01 ;X2 = 8.87, P 〈 0.005, respectively）. The individuals with AA genotype and A allele were 3.25 （95% CI： 1.28 - 8.28, P 〈 0.05 ） and 1.99 （ 95% CI： 1.28 -3.08 ,P 〈 0.01 ） times susceptible to asthma compared with CC genotype and C allele. The serum concentration of IL-10 in asthma group was significantly lower than that in healthy control group,whether in attacking-stage or remission-stage, and there were significant differences （ all P 〈 0.01 ）. The individuals with AA genotypes had lower serum IL-10 concentration than those with CC genotypes （P 〈 0.05 ） o Conclusions The IL-10 gene - 592C/A polymorphism is different significantly between children with bronchial asthma and healthy ones, and this polymorphism influences the concentration of IL-10. The individuals with AA genotype have relatively lower IL-10 concentration, and A allele may be one of genetic susceptibility factor of bronchial asthma in children.
出处
《中华实用儿科临床杂志》
CAS
CSCD
北大核心
2013年第4期261-264,共4页
Chinese Journal of Applied Clinical Pediatrics
