摘要
目的:探讨青蒿琥酯(Artesunate,Art)对大鼠肝星状细胞(HSC-T6)增殖和凋亡的影响。方法:选用活性良好的HSCT6细胞进行体外培养,应用倒置显微镜观察药物作用后的细胞形态改变;用CCK-8测定不同浓度、不同时间Art对该细胞增殖的抑制作用的影响;用AnnexinⅤ和PI双染的流式细胞术检测HSC-T6的细胞凋亡率的影响。结果:①倒置显微镜结果显示,Art作用过的贴壁细胞数目变少、突触变短变细、细胞表面变皱、由星形变成圆形。②12.5～200μg·ml^(-1)的Art在0～72 h范围内对HSC的增殖有明显的抑制作用(P<0.01),抑制率有时间和剂量依赖性。③流式细胞仪结果显示随着剂量、时间的增加,凋亡率显著增加(P<0.05)。结论:Art能抑制HSC-T6细胞的增殖,并可促进细胞凋亡。
Objective：To study the effects of artesunate（Art） on the proliferation and apoptosis of rat hepatic stellate cells T6 （HSC-T6）.Method：HSC-T6 was cultured in vitro.Morphological changes of HSC-T6 after the drug treatment were observed by an inverted microscope.The effects of Art with different concentrations on the proliferation of HSC-T6 were detected at different times by CCK-8.A flow cytometry with double staining of Annexin and PI was used to detect the apoptosis rate of HSC-T6.Result：The results shown by the inverted microscope suggested the number decrease of the adherent cells with shorter and thinner synapses,and the cell surface wrinkled and the shape changed from star to round.Art with the concentration of 12.5-200μg·ml^（-1） during 0-72 h exhibited notable anti-proliferative effect on HSC-T6（P 0.01） with time-and dose-dependence.The results of flow cytometry showed that the apoptosis rate of HSC-T6 was significantly enhanced with the increase of the Art dose and the acting time（P 0.05）.Conclusion：Art can inhibit the proliferation and promote the apoptosis of HSC-T6.
出处
《中国药师》
CAS
2013年第3期322-325,共4页
China Pharmacist
基金
湖北省自然科学基金项目(编号:2011CDB491)
