摘要
Ｂｅｌ－７４０２人肝癌细胞在ＤＤＢ（１０￣（－４）ｍｏｌ·Ｌ￣（－１））处理后，生长和克隆形成受到明显抑制，电子显微镜观察到ＤＤＢ作用过的细胞核仁减少或消失，核与胞浆比例缩小。Ｂｅｌ－７４０２细胞内环化腺苷酸（ｃＡＭＰ）和钙调蛋白（ＣａＭ）的含量，经ＤＤＢ处理不同时间后皆显示明显高于对照组。此外，ＤＤＢ还能降低从Ｂｅｌ－７４０２细胞内提取出的ＤＮＡ拓扑异构酶ＩＩ（ＴｏＰｏＩＩ）活力。说明ＤＤＢ对Ｂｅｌ－７４０２人肝癌细胞的作用机理与ｃＡＭＰ，ＣａＭ和ＴｏＰｏＩＩ有关。
DDB is a hepatoprotectant and has been widely used for the treatment of chronicviral hepatitis in China,The drug markedly improved the abnormal liver ft1nction particularly inlowering the elevated serum transaminases in patients.It is k nown that there is a close correlationbetween primary hepatocarcinoma and chronic viral hepatitis.The aim of the present study is toevaluate the effect of DDB on hepatocarcinoma cell line.The results showed that the growth andclonogenicity of Bel-7402 human hepatocarcinoma cell line cuItured with DDB were markedlyinhibited.The nucleoles of the cells treated with DDB disappeared or their numbers and nucleus/cytoplasm ratio decreased under electron microscopic observation, DDB at the concentration of 10-mol·L-1 significantly increased the contents of cAMP and calmodulin(CaM)in Bel-7402 hepatocarci-noma cells.DDB was also found to inhibit topoisomerase II activity of Bel-74 02 hepatocarcinomacells.These results suggest that the mechanism of inhibition of DDB on several phenotypes of Bel-74 02 cell line may be related to its effect on cAMP and CaM content as well as topoisomerase IIactivity.
出处
《药学学报》
CAS
CSCD
北大核心
1996年第2期101-106,共6页
Acta Pharmaceutica Sinica
关键词
肝癌细胞株
联苯双酯
钙调蛋白
cAMP
抗癌作用
Dimethyl-4,4'-dimethoxy-5,6,5',6'-dimethylene dioxybiphenyl- 2,2'-dicarboxylate(DDB)
Human hepatocarcinoma cell line
cAMP
CaM
Topoisomerase II
