摘要
目的探讨中药肝复康通过Raf/ERK介导的信号转导通路治疗肝纤维化大鼠的作用机制。方法制备四氯化碳诱导的肝纤维化大鼠,应用肝复康干预;采用RT-PCR法测定肝组织及HSC-T6细胞Raf、ERK和Ⅲ型胶原mRNA水平的变化;采用免疫组织化学法检测肝组织ERK的表达。结果模型组肝组织Raf、ERK和Ⅲ型胶原mRNA水平较正常对照组明显上调(P<0.01),而肝复康治疗组则明显下调(P<0.01);模型组肝组织ERK蛋白的表达上调,而肝复康治疗组表达则下调。结论肝复康对肝纤维化有明确的治疗作用,其抗肝纤维化的作用机制可能与ERK介导的信号转导通路有关。
Objective To investigate the mechanisms by which Gan-fu-kang inhibiting Raf/ERK signal transduction pathway in rats with hepatic fibrosis.Methods The rats with liver fibrosi were made by subcutaneously injecting with 10%CCl4 and treated with Gan-fu-kang.Expression of Raf,ERK and collagen Ⅲ mRNA were detected by RT-PCR.The ERK expression in liver tissues were detected by immunohistochemistry staining.Results The levels of Raf,ERK and collagen Ⅲ mRNA in model group increased than in control group（P0.01）;In the immunohistochemistry staining,the expression of ERK protein in model group was obviously elevated than in Gan-fu-kang treatment group.Conclusion Ganfukang has effective treatment on liver fibrosis and its treatment mechanisms probably involved in ERK-induced signal transduction pathway.
出处
《实用肝脏病杂志》
CAS
2012年第2期108-110,共3页
Journal of Practical Hepatology
基金
辽宁省教育厅高等学校科研项目(编号:2009A190)
