摘要
目的:探讨青蒿琥酯抗肝纤维化的作用机制。方法:用MTT法观察青蒿琥酯对HSC增殖的影响,用流式细胞仪检测HSC在细胞周期中DNA含量的改变,测定不同浓度青蒿琥酯作用于HSC后上清液中Hpy、LDH的含量。结果:1.5~100μg/m l青蒿琥酯在0~72h范围内对HSC增殖有抑制作用,并有药物浓度时间依赖关系。各青蒿琥酯处理组中G0/G1期细胞比例逐渐增高,与正常组相比由56.2±1.3%升至72.6±2.3%左右;S期比例逐渐降低,由27.1±2.6%降至13.8±4.1%左右,而对G2/M期无影响。青蒿琥酯(12.5μg/m l^100μg/m l)组上清液Hyp值逐渐减小,呈剂量依赖关系,与相应的空白对照组比较有显著性差异;青蒿琥酯组上清液LDH与空白对照组比较无显著性差异。结论:青蒿琥酯能阻止HSC从G0/G1期进入S期,体外能明显抑制HSC的增殖,减少胶原的分泌,且在实验观察剂量范围内对HSC没有细胞毒性。
Objective： To explore the mechanism that artesunate could inhibit hepatic fibrosis. Methods： The inhibition of cell proliferation was determined by MTT method. Cell cycle was determined by flow cytometry analysis. The content of hyroxyproline was measured by colorimetric method. Cytotoxicity was measure by LDH colorimetry. Results： Artesunate markedly inhibited the proliferation with the concentration of 1.5～100 μg/ml in dose-dependent manner. Flow cytometry analysis showed that artesunate could inhibit DNA synthesis of S phase, block the cell cycle beyond G1 phase, which existed dose-dependent relation. Percentage concentration of DNA in G0/G1 phase was gradually increased （from 56.2±1.3% to 72.6±2.3%） and that of S phase was decreased （from 27.1±2.6% to 13.8±4.1%）. The content of hyroxyproline was gradually decreased along with artesunate concentration of from 12.5 μg/ml to 100 μg/ml in dose-dependent relation compared with control group （P 〈 0.05）. The content of LDH of every artesunate group was not obvious compared with control group. Conclusions： Artesunate could inhibit the proliferation of HSCs, and interfered with HSCs entrance into S phase from G0/G1 phase.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2006年第3期25-27,共3页
Pharmacology and Clinics of Chinese Materia Medica
