摘要
目的探讨HPV L1蛋白在宫颈脱落细胞病变中的表达情况及其临床意义。方法以非同位素标记核酸分子杂交技术和免疫组织/细胞化学技术为基础,在同时采用核酸和免疫水平双重检测传染病病原体人乳头瘤病毒壳蛋白抗体(HPV L1)的方法对124例妇科宫颈脱落细胞TCT标本进行HPV L1蛋白检测。结果 HPV L1蛋白的总阳性率为44.35%(55/124)。其中轻度炎症的阳性率为66.67%(26/39);中度炎症的阳性率为41.94%(13/31);重度炎症的阳性率为37.50%(6/16);低级别上皮内瘤变的阳性率为30.43%(7/23);高级别上皮内瘤变的阳性率为20.00%(3/15)。HPV L1蛋白在轻度炎症的阳性表达率高于中度炎症和重度炎症,明显高于低级别上皮内瘤变和高级别上皮内瘤变,差异具有统计学意义(χ2=13.65,P<0.05)。结论 HPV L1蛋白的阳性表达与宫颈脱落细胞病变的类型密切相关,随着宫颈脱落细胞病变的加深,HPV L1蛋白的阳性率逐渐减弱,HPV L1蛋白的丢失可能是宫颈病变进展的一个早期事件,HPV L1蛋白的阳性表达与宫颈病变的生物学行为密接相关,可作为宫颈病变治疗预后的可靠理论依据。
Objective To determine the expression of HPV L1 protein in exfoliated cervical cells and discuss its clinical significance.Methods The expression of HPV L1 protein in cervical tissue Thinprep cytology test（TCT） specimens of 124 patients were detected by using isotope labeling nucleic acid molecular hybridization and immune tissue/cell chemical technology.Results The total positive rate of HPV L1 protein in exfoliated cervical cells was 44.35%（55/124）.The positive rate in mild inflammation was 66.67%（26/39）,in the moderate inflammation was 41.94%（13/31）,in the severe inflammation was 37.50%（6/16）,in low-grade intraepithelial neoplasia was 30.43%（7/23）,in high-grade intraepithelial neoplasia was 20%（3/15）.The positive rate in the mild inflammation was higher than that in moderate and severe inflammation,and was much higher than that in low-and high-grade intraepithelial neoplasia（χ2=13.65,P0.05）,the difference was statistical significant.Conclusion The positive expression of HPV L1 protein was closely related to the lesions types of exfoliated cervical cells.With the deepening of cervical lesions,the positive rate of HPV L1 protein decreased gradually.The loss of HPV L1 protein may be an early event of the cervical lesions progress.The positive rate of HPV L1 protein was correlated with its biological behavior of cervical lesion.It can be used as cervical lesion treatment outcome and reliable theoretical basis.
出处
《中华全科医学》
2013年第3期394-395,F0003,共3页
Chinese Journal of General Practice
关键词
宫颈脱落细胞病变
HPVL1蛋白
核酸和免疫水平双重检测
Exfoliated cervical cells
Lesions of the cervix
HPV L1 protein
Nucleic acids hybridization
Immunohistochemistry
