摘要
目的调查和分析2013年-2015年舟山地区妇女的人乳头状瘤病毒(HPV)E6/E7 mRNA阳性率变迁。方法选取2013年1月-2015年12月在本院就诊的本地常住妇女503例,采集宫颈脱落细胞,荧光定量PCR法检测HPV E6/E7 mRNA表达,以HPV E6/E7 mRNA>103为阳性。结果共计HPV E6/E7 mRNA阳性88例,总阳性率为17.49%。≤30岁组中,2015年的HPV E6/E7 mRNA阳性率高于2014年,差异有统计学意义(χ~2=4.08,P<0.05),≥41岁组中,2015年的HPV E6/E7 mRNA阳性率高于2014年,差异有统计学意义(χ~2=8.92,P<0.01)。2015年的乡村组HPV E6/E7 mRNA阳性率高于2014年,差异有统计学意义(χ~2=11.14,P<0.01)。结论舟山地区≥41岁和乡村妇女是HPV E6/E7 mRNA阳性高发人群,有必要对其加强宫颈癌病变监控。
Objective To investigate the trend of human papillomavirus（HPV） E6/E7 gene in women from Zhoushan.Methods 503 local clinical women were selected for the collection of cervical cells from January 2013 to December 2015.HPV E6/E7 gene expression was detected by quantitative real-time PCR.Higher than 103 copies of mRNA expression was set as positive.Results Total positive rate of HPV E6/E7 gene expression was 17.49%（88/503）.In ≤30 age group,the positive rate of HPV E6/E7 gene in 2015 was significantly higher than 2014,with the differences statistically significant（χ^2= 4.08,P〈0.05）.In ≥41 age group,positive rate of HPV E6/E7 gene in 2015 was significantly higher than 2014,with the differences statistically significant（χ^2= 8.92,P〈0.01）.In village group,positive rate of HPV E6/E7 gene in 2015 was significantly higher than 2014,with the differences statistically significant（χ^2= 11.14,P〈0.01）.Conclusion Rural women ≥ 40 in Zhoushan is a high incidence group of HPV mRNA E6/E7,and it is necessary to strengthen the monitoring of cervical cancer lesions.
作者
周晓
杜玲
ZHOU Xiao DU Ling(Zhoushan Maternal and Child Health Care Hospital, Zhoushan, Zhejiang 316004, China)
出处
《中国卫生检验杂志》
CAS
2017年第4期588-589,593,共3页
Chinese Journal of Health Laboratory Technology
