摘要
目的了解川南地区医院感染产β-内酰胺酶(ESBLs)大肠埃希菌基因型特征和临床耐药情况。方法收集2010年10月～2011年9月期间本院住院患者分离的大肠埃希菌,双纸片协同试验确定产ESBLs大肠埃希菌,PCR扩增技术进行基因分型,K-B法比较产ESBLs阳性株与阴性株耐药率。结果 77株大肠埃希菌双纸片协同试验确认28为株产ESBLs菌株;PCR扩增显示,28株产ESBLs大肠埃希菌中TEM型11株(39.29%),SHV型6株(21.43%),TEM、SHV双基因型2株(7.14%),非TEM、非SHV型9株(32.14%)。K-B法测定ESBLs阳性株对多种抗生素的耐药率(亚胺培南除外)高于ESBLs阴性株。结论川南地区产ESBLs大肠埃希菌以TEM型为主,对常用抗菌药物耐药率高于ESBLs阴性菌株。
Objective To investigate genotypes and clinical drug resistance of ESBL producing Escherichia coli in hospi-tals in southern Sichuan. Methods E. coli was collected from October 2010 to September 2011. ESBLs were detected using a double disk synergy test, PCR was used to determine the genotype of the ESBLs, and resistant strains that were positive and negative for ESBLs were tested by the Kirby-Bauer method. Results There were 28 strains of ESBL-pro-ducing E. coli, including 11 strains （39.29 %） producing TEM beta-lactamases, 6 strains （21.43%） producing SHV be-ta-lactamases, 2 strains （7. 14%） producing both TEM and SHV beta-lactamases, and 9 strains （32. 14%） producing neither TEM nor SHV beta-lactamases. ESBL-produeing E. coli had a significantly higher rate of multi-drug resistance （excluding imipenem） compared to strains that were ESBL-negative. Conclusion In southern Siehuan, ESBL-producing E. coli produced primarily TEM beta-lactamases. ESBL-producing E. coli had a significantly higher rate of resistance than strains that were ESBL-negative.
出处
《中国病原生物学杂志》
CSCD
北大核心
2013年第2期123-125,共3页
Journal of Pathogen Biology
基金
四川省重点学科重点建设项目(No.SZD0241)
关键词
大肠埃希菌
超广谱Β-内酰胺酶
基因型
Escherichia coli
extended spectrumbeta-lactamases （ESBLs）
genotype
