摘要
采用膜片钳全细胞记录和“Y型管”技术 ,观察七氟烷 (0 .38× 10 - 3 ～ 3× 10 - 3 m ol/L )对单个培养 SD鼠背根神经节细胞 3× 10 - 6mol/Lγ-氨基丁酸 (GABA )调控的氯电流影响 ,实验结果发现 ,0 .38× 10 - 3 ,0 .76× 10 - 3 ,1.5 2× 10 - 3 ,2 .2 8× 10 - 3 ,3.0 4× 10 - 3 mol/L七氟烷分别增强氯电流峰值高度至对照值的 (149± 2 5 ) % ,(2 0 3± 2 7) % ,(32 7± 79) % ,(331± 10 9) % ,(2 43± 71) %。实验结果提示 ,临床麻醉浓度的七氟烷能增强培养鼠背根神经节细胞 3× 10 - 6m ol/L
The whole cell patch clamp technique and "Y tube" method were applied to evaluate the effects of sevoflurane (from 0.38×10 -3 to 3×10 -3 mol/L) on chloride current induced by bath utilization of 3×10 -6 mol/L γ aminobutyric acid (GABA) in the single cultured rat dorsal root ganglia neurons. Experimental data demonstrated that when peak amplitude of chloride current induced by 3×10 -6 mol/L GABA was considered as 100 % in the presence of 0.38×10 -3 , 0.76×10 -3 , 2.28×10 -3 , 3.04×10 -3 mol/L sevoflurane peak amplitude of chloride current was rose to (149±25) %, (203±27) %, (327±79) %, (331±109) %, (243±71) % correspondingly. This finding suggests that sevoflurane, at concentrations relevant clinical anesthesia, can enhance GABA mediated chloride current in sensory neurons.
出处
《同济医科大学学报》
CSCD
2000年第5期458-460,共3页
Acta Universitatis Medicinae Tongji
关键词
七氟烷
Γ-氨基丁酸
氯电流
背根神经节细胞
sevoflurane
γ aminobutyric acid
patch clamp technique
chloride current
