摘要
采用高效液相色谱-串联质谱技术,多重反应监测模式对岩茶水库岭肉桂样品中的原花青素组分进行定性和定量分析。采用Waters SunFire-C18柱(150mm×2.1mm×5μm),流动相为乙腈和0.1%甲酸水溶液,流速0.25mL/min,柱温25℃,进样量3μL。结果表明,岩茶水库岭肉桂样品中的原花青素主要有儿茶素、表儿茶素、表儿茶素没食子酸酯和原花青素B2,其回归方程和相关系数分别为:y1=642.48x-63.693,r=0.999 5;y2=550.45x+10.386,r=0.999 6;y3=2 261.5x-1 320.0,r=0.999 5和y4=4 598.5x-4 079.0,r=0.999 5;其线性范围为5.00～150.00mg/L。方法的加标回收率为93.9%～108.0%,变异系数小于7.53%,最低检出限为0.005 5～0.013 2μg/g。
The procyanidins in the rock tea shuikuling rougui were identified and determined by liquid chromatography-tandem mass spectrometry（HPLC-ESI-MS/MS） technique with the multiple-reaction monitoring mode. The catechins, epicatechin, epicatechin gallate and procyanidins B2 were separated and identified successfully by Waters SunFire-C18 column （150 mm×2.1 mm×5 μm）, with 0. 1% formic acid solution and acetonitrile as the mobile phase in gradient elution, flow velocity was 0.25 mL/min, column temperature was 25 ℃, and sample volume was 3 μL. The linear regression equation and correlation coefficient of the catechins, epicatechin, epicatechin gallate and procyanidins B2 are y1=642.48x- 63.693, r=0. 999 5; y2 =550.45x＋10. 386, r=0. 999 6; y3=2 261.5x-1 320.0, r=0.999 5 and y4 =4 598. 5x-4 079. 0, 5.00-150. less than 7. 00 mg/L, the 53% and the recoveries are r = 0. 999 5, respectively. The linear ranges are 93. 9%-108.0%, the coefficients of variation are limit of detection are 0. 005 5-0. 013 2 μg/g.
出处
《质谱学报》
EI
CAS
CSCD
北大核心
2013年第1期51-56,共6页
Journal of Chinese Mass Spectrometry Society
基金
辽宁省教育厅科学技术基金课题(L2010001)资助
关键词
岩茶水库岭肉桂
原花青素
高效液相色谱-串联质谱法
rock tea shuikuling rougui
procyanidins
liquid chromatography-tandem massspectrometry（HPLC-ESI-MS-MS）
