摘要
【目的】文章旨在研究植物乳杆菌(Lactobacillus plantarum)C88的抗氧化作用。【方法】采用0.1mmol·L-1和0.2 mmol·L-1浓度H2O2连续处理Caco-2细胞12—48 h造成氧化损伤,通过测定细胞培养液上清和细胞裂解液的自由基清除能力及抗氧化物酶活性,评价植物乳杆菌C88对Caco-2细胞抗氧化损伤的保护作用。【结果】与氧化损伤模型组相比,植物乳杆菌C88在1011CFU/mL浓度下,能显著提高细胞裂解液的谷胱甘肽过氧化物酶(GSH-Px)活性(P<0.05)和总抗氧化(T-AOC)能力(P<0.05);以及细胞裂解液的羟自由基清除率(P<0.05)、谷胱甘肽过氧化物酶(GSH-Px)(P<0.05)和超氧化歧化酶(SOD)活性。细胞裂解液的羟自由基清除率低于氧化应激对照组。【结论】植物乳杆菌(L.plantarum)C88通过提高Caco-2细胞的自由基清除能力和抗氧化酶活性,表现出了较强的抗氧化活性。
[ Objective] The aim of the study was to evaluate the antioxidant activity ofL. plantarum C88 against H202 induced Caco-2 cell oxidative stress. [Method] In vitro methods simulating gastric and small intestine digestive processes, coupled with human colon carcinoma cells in oxidative stress, to hydrogen peroxide at concentrations of 0.1 mmol·L^-1 and 0.2 mmol·L^-1 for 12 h, 24 h, 36 h, 48 h, are the valid tools to evaluate the antioxidant activity ofL. plantarum C88. The free radical scavenging capacity and antioxidant enzymes activitties in the cell culture supematants and cell disruptions were measured. [Result] Compared with the oxidative stress group, L. plantarum C88 at 10UCFU/mL significantly increased the glutathione peroxidase （GSH-Px） （P〈0.05） and the total antioxidation capacity （T-AOC） （P〈0.05） in the cultured supernatants, the hydroxyl radical scavenging ability （P〈0.05）, the glutathione peroxidase （GSH Px） （P〈 0.05） and the superoxide dismutase （SOD） activity in the cell disruptions. [ Conclusion ] Results of the study suggested that L. plantarum C88 could protect the Caco-2 cells against H202-induced oxidative stress by renewing the enzymatic and non-enzymatic antioxidant defense system.
出处
《中国农业科学》
CAS
CSCD
北大核心
2013年第3期606-613,共8页
Scientia Agricultura Sinica
基金
国家自然科学基金项目(31071574)
农业部现代农业产业技术体系专项资金(CARS-37)
吉林省科技发展计划项目(20115026)
关键词
乳酸菌
植物乳杆菌
抗氧化活性
益生性
lactic acid bacteria
Lactobacillus plantarum
antioxidant activity
probiotic
