摘要
为研究脱落酸(ABA)对蝴蝶兰抗寒性的影响,以蝴蝶兰中苗为试材,叶面喷施50 mg.L-1脱落酸(ABA)1 d后,于6℃低温胁迫4 d,以清水喷施为对照,在低温处理0,1,2,3和4 d后测定叶片相对电导率、丙二醛(MDA)含量、脯氨酸(Pro)含量、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性。结果表明:喷施ABA可以延缓蝴蝶兰叶片的相对电导率和MDA含量的上升,减少Pro的累积量,提高CAT和SOD的活性。说明喷施ABA提高了蝴蝶兰叶片的抗氧化酶活性,减少了膜脂过氧化产物的积累,降低了细胞膜透性,从而减轻了低温胁迫的伤害。
The study was to reveal effects of exogenous ABA on chilling resistance of Phalaenopsis seedlings under low temperature stress. Leaves were sprayed with 50 mg· L^- 1 ABA under room temperature for 1 day, and then plants were treated under 6℃ for 4 days. Plants treated with water were used as the control. Relative electric conductivity （REC）, malondialdehyde （MDA） and proline content, and activities of catalase （CAT） and superoxide dismutase （SOD） were determined on four days after cold treatment, The results showed that ABA treatment delayed the in- crease of REC and MDA content, reduced the accumulation of proline, and increased the activities of SOD and CAT. These above results indicated ABA treatment could increase the activities of antioxidant enzymes, reduce the reaction of membrane lipid peroxidation and the infiltration of membrane, and thus decrease the damage under low tempera- ture in Phalaenopsis plants.
出处
《浙江农业学报》
CSCD
北大核心
2013年第1期68-72,共5页
Acta Agriculturae Zhejiangensis
基金
浙江省重大科技专项资助项目(2009C12095)
浙江省科技资助项目(2010C32052)
关键词
ABA
蝴蝶兰
低温胁迫
相对电导率
丙二醛
脯氨酸
抗氧化酶
ABA
Phalaenopsis
low temperature
relative electric conductivity
malondialdehyde
proline
antioxi- dant enzymes
