摘要
目的 以昆明小鼠作实验动物模型 ,探讨猪囊虫病基因工程疫苗的免疫效果。方法 78只昆明小鼠随机分为 3组 ,第一组用猪囊虫病基因工程疫苗免疫 1次 ,每二组免疫 2次 ,第三组不免疫。第一次免疫后 2 0 d各小鼠经尾静脉感染已孵化的有活力的六钩蚴 ,6 3d后剖检小鼠检查有无囊虫 ,并观察虫体的活力。用免疫学方法检测血清中囊虫抗原和抗体 ,以监测疫苗的免疫反应及虫体寄生状况。结果 所有免疫小鼠均未出现不良反应 ;免疫 7d后部分免疫小鼠可检测到囊虫抗体 ,免疫后2 1d所有免疫小鼠的囊虫抗体均呈阳性。未免疫的第三组各小鼠均检出囊虫 ,共 6 5个 ,主要寄生在胸腔 ;第一组和第二组分别有 2只和 1只小鼠检出囊虫 ,分别为 2个和 1个 ,保护率分别为 96 .9%和 98.4%。CA检测结果与剖检结果一致 ,检出囊虫的小鼠 ,其 CA均呈阳性。结论 用小鼠动物模型可进行猪囊虫病免疫预防试验 ,猪囊虫病基因工程疫苗安全性好 。
Aim\ To investigate the immune effects of the genetic engineering vaccine against swine cysticercosis with the animal mode of kunming mouse.Method\ 78 kunming mice were randomly divided into three groups:1)injection one times with the vaccine,2)injection twice with the vaccine at intervals of ten days,3)non-vaccination group.All the mice were infected with the vial hatched oncopheres by the tag vein at 20 days after the first vaccination,and were killed and dissected to look for swine cysticerci 63 days later.In order to know the immune response of the vaccine and the stateof parasites in the rats,the antigens and antibodies of swine cysticerci were detected by immune methods.Results\ The vaccine was safety to all the vaccinated rats.The antibodies against swine cysticerci began to appear positive in some of the immunized mice at 7 days postvaccination,and they were all positive at 21 days.65 cysticerci were found in the mice of the nonvaccination group,which lodged mainly in the chests.2 and 1cysticerci were respectively found in two rats of the first group and in one rat of the second group,their protective rates of cysticerci were 96.9% and 98.4%,respectively.Conclusion\ Kunming mouse as the animal model of swine cysticercosis was stable and reliable,and the genetic engineering vaccine against swine cysticercosis was safety and efficiency.
出处
《中国人兽共患病杂志》
CSCD
北大核心
2000年第6期56-57,60,共3页
Chinese Journal of Zoonoses
关键词
猪囊虫病
动物模型
免疫
基因工程疫苗
Swine cysticercosis
animal model
vaccination
the genetic engineering vaccine
