摘要
目的 :研究茶多酚对致龋菌在唾液获得性膜粘附的影响 ,进一步探讨茶多酚的防龋机制。方法 :采用唾液包被羟磷灰石 (S HA)形成实验性膜的体外模式 ,以变形链球菌和粘性放线菌作为主要致龋菌 ,用茶多酚分别处理S HA和细菌 ,观察细菌在S HA粘附的情况。结果 :两组实验中 ,茶多酚溶液浓度为 1～ 4mg/ml时都能显著抑制变形链球菌和粘性放线菌在S HA上的粘附 ,且抑制作用随茶多酚溶液浓度的升高而逐渐增强。结论 :茶多酚能有效抑制主要致龋菌对唾液获得性膜的粘附。
Objective: It has been proved that tea polyphenols (TP) can inhibit the sucrose-dependent adherence of oral cariogenic bacterium to the tooth surface. This study is carried on to investigate the effects of tea polyphenols on the preliminary adherence of main cariogenic bacterium to salivary acquired pellicle and reveal the anticarious mechanisms of tea polyphenols further. Methods: The hydroxyapatites were coated by saliva to form S-HA, an in-vitro model of experimental pellicle. Streptococcus mutans 3a\-3 and Actinomyces viscosus ATCC 19246 were chosen as the main cariogenic bacterium, and the microorganisms were radiolabelled by growing them in TPY culture medium supplemented with \+3H-thymidine. Tea polyphenols solution was prepared, the concentrations of which were 0 5, 1 0, 2 0 and 4 0mg/ml respectively. Two parts of inhibited adherence tests were done with TP-treated S-HA or TP-treated bacterium. In the first one, S-HA was immerged into the TP solution of different concentrations for 1h before the attachment of bacterium. In the other, the bacterium were mixed with the TP solution for 1h and then centrifuged and washed before bound to S-HA. The whole process was carried on in KCl buffer solution. The number of \+3H-thymidine-labelled bacterium which attached to S-HA were determined by scintillation counting. Results: In both the two parts of this study, tea polyphenols solution, the concentrations of which were changed from 1 0 to 4 0mg/ml, could decrease the number of S. mutans 3a\-3 and A. viscosus ATCC 19246 , and the inhibition rates rose with the increase of the concentrations of TP solution. But the inhibitory effects of 0 5mg/ml TP solution were inconsistent in different tests. The results showed that 1 0～4 0 mg/ml TP solution could inhibit the attachments of S. mutans and A. viscosus to S-HA effectively. The inhibitory functions of tea polyphenols were so obvious that in some experiments, while the concentrations of TP solution were changed from 2 0 to 4 0mg/ml, the number of \+3H-thymidine-labelled bacterium which attached to S-HA was even less than that of the same bacterium which attached to naked HA. Conclusion: Tea polyphenols can inhibit the preliminary adherence of Streptococcus mutans and Actinomyces viscosus to S-HA effectively. TP may prevent enamel from caries by decreasing the adherence of main cariogenic bacterium to salivary acquired pellicle.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2000年第5期336-339,共4页
West China Journal of Stomatology
关键词
茶多酚
致龋菌
唾液获得性膜
粘附
龋齿
tea polyphenols\ \ cariogenic bacteria\ \ salivary acquired pellicles\ \ adherence
