摘要
目的 :研究茶多酚对致龋菌在胶原粘附的影响 ,进一步探讨茶多酚的防龋机制。方法 :采用胶原溶液包被羟磷灰石 (C HA)形成实验性膜的体外模式 ,以变形链球菌、粘性放线菌和乳杆菌作为主要致龋菌 ,用茶多酚分别处理C HA和细菌 ,观察细菌在C HA粘附的情况。结果 :在两组实验中 ,1～ 4mg/ml的茶多酚溶液都能显著抑制变形链球菌、粘性放线菌和乳杆菌在C HA上的粘附 ,且抑制作用随茶多酚溶液浓度的升高而逐渐增强。结论 :茶多酚能有效抑制主要致龋菌对胶原的粘附。
Objective: In the first phase of our study, it has been proved that tea polyphenols (TP) can inhibit the preliminary adherence of oral cariogenic bacterium to salivary acquired pellicle. This study is carried on to investigate the effects of tea polyphenols on the preliminary adherence of main cariogenic bacterium to collagen I and reveal the anticarious mechanisms of tea polyphenols further. Methods: Tea polyphenols solution was prepared firstly, the concentrations of which were 0 5, 1 0, 2 0 and 4 0mg/ml. An in-vitro model of experimental pellicle(C-HA) was formed by immerging the hydroxyapatites in 250 μg/ml collagen I solution for 1h. Streptococcus mutans 3a\-3, Actinomyces viscosus ATCC 19246 and Lactobacillus ATCC 4546 were chosen as the main cariogenic bacterium and radiolabelled by growing them in TPY culture medium supplemented with \+3H-thymidine. Two parts of inhibited adherence tests were done with TP-treated C-HA or TP-treated bacterium respectively. In the first one, C-HA was immerged into the TP solution of different concentrations for 1h before the attachment of bacterium. In the other, the bacterium were mixed with the TP solution for 1h and then centrifuged and washed before bound to C-HA. KCl buffer solution provided a steady condition to the whole process. Finally, the number of \+3H-thymidine-labelled bacterium which attached to C-HA were determined by scintillation counting. Results: In both the two parts of this study, tea polyphenols solution, the concentrations of which were changed from 1 0 to 4 0mg/ml could inhibit the attachments of S. mutans 3a\-3, A. viscosus ATCC 19246 and Lactobacillus ATCC 4546 to C-HA effectively, and the inhibition rates rose with the increase of the concentrations of TP solution. But the inhibitory effects of 0 5mg/ml TP solution were inconsistent in different tests. The inhibitory functions of tea polyphenols were so prominent that in some experiments, while the concentrations of TP solution were higher than 2 0mg/ml, the number of \+3H-thymidine-labelled bacterium which attached to C-HA was even less than that of the same bacterium which attached to naked HA. These results showed that TP had the remarkable inhibitory efficiency on the attachments of S. mutans, A. viscosus and Lactobacillus to C-HA.Conclusion: Tea polyphenols can inhibit the preliminary adherence of Streptococcus mutans, Actinomyces viscosus and Lactobacillus to C-HA effectively. TP may prevent dentine from caries by decreasing the adherence of main cariogenic bacterium to collagen.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2000年第5期340-342,共3页
West China Journal of Stomatology
关键词
茶多酚
致龋菌
胶原
粘附
龋齿
tea polyphenols\ \ cariogenic bacteria\ \ collagen\ \ adherence
