摘要
目的 探讨大鼠消化道是否存在 Gn RH受体 m RNA及其定位。 方法 用原位杂交组织化学法。 结果 胃底腺壁细胞、胃小凹上皮细胞可检测到较强的 Gn RH受体 m RNA杂交信号。3段小肠绒毛上皮细胞、小肠腺细胞可检测到较强的 Gn RH受体 m RNA杂交信号。盲肠、结肠和直肠的粘膜上皮细胞、肠腺上皮细胞也能检测到 Gn RH受体 m RNA杂交信号。信号物质均分布在胞质内 ,胞核呈阴性。 结论 大鼠消化道能合成 Gn RH受体。Gn RH也是一种胃肠激素 ,它由消化系统自身合成 。
Objective\ To study whether gonadotropin releasing hormone receptor(GnRHR) mRNA exists in rat digestive tract and it's cellular localization. Methods\ In situ hybridization using digoxigenin\|labeled oligonucleotide probes was performed. Results\ Parietal cells of gastric gland and epithelium of gastric pit were detected to have GnRHR mRNA hybridization signal. In small intestine, the villou' s epithelial cells and glandular cells could be detected to have strong GnRHR mRNA hybridization signal. In caecum、colon、rectum,very strong GnRHR mRNA hybridization signal was also detected in mucosa epithelium and glandular epithelium.The signals distributed in cytoplasm of all positive cells with negative nuclei. Conclusion\ The GnRHR mRNA was widely distributed in rat digestive tract with distributive pattern similar to those of GnRHR in pituitary. It suggests that the GnRH is produced by digestive system and response for modulating the digestive function as a gastrointestinal hormone.\;
出处
《解剖学报》
CAS
CSCD
北大核心
2000年第3期250-252,I010,共4页
Acta Anatomica Sinica
基金
国家自然科学基金!( 3 9770 3 88)
