摘要
目的探讨大鼠消化道是否存在ＧｎＲＨ受体及其定位。方法采用兔抗ＧｎＲＨ抗独特型抗体的免疫组织化学ＡＢＣ法。结果胃底腺壁细胞、胃小凹上皮细胞及肌间神经丛的副交感神经节细胞呈ＧｎＲＨ受体免疫反应性。三段小肠绒毛上皮细胞、潘氏细胞、十二指肠腺上皮细胞以及小肠肌间神经丛副交感神经节细胞，均呈较强的ＧｎＲＨ受体免疫反应性。在盲肠、结肠和直肠，粘膜上皮细胞、肠腺上皮细胞及肌间神经丛呈ＧｎＲＨ受体免疫反应性。阳性物质均分布在胞质内，胞核呈阴性。结论大鼠消化道广泛分布有ＧｎＲＨ受体，其分布模式与先前ＧｎＲＨ及ＧｎＲＨ－ｍＲＮＡ的相类似。说明胃肠道的一些细胞分泌的ＧｎＲＨ可能以自分泌或旁分泌的方式参与消化功能的调节。
Objective\ To study whether gonadotropin releasing hormone receptor(GnRHR) can exist in rat digestive tract and its localization.Method Immunohistochemical ABC method was used to study the localization of GnRHR in rat digestive tract.Results Parietal cells of gastric gland,epithelium of gastric pit and parasympathetic ganglion cells of myenteric plexus show GnRHR immunoreactivity.In small intestine,the villous epithelial cells,Paneth cells and parasympathetic ganglion cells in myenteric plexus show GnRHR immunoreactivity.In caecum colon rectum,the surface epithelium,glandular epithelium and parasympatheitc ganglion cells also show GnRHR immunoreactivity.The reactive substance distributes in cytoplasm of all positive cells with negative nuclei.Conclusion The GnRHR is widely distributed in rat digestive tract with distributive pattern similar to those of GnRH and GnRH mRNA.It suggests that the GnRH may response for modulating the digestive function via autocrine or paracrine mechanism.
出处
《解剖学报》
CAS
CSCD
北大核心
1999年第2期152-154,I011,共4页
Acta Anatomica Sinica
基金
国家自然科学基金
关键词
GNRH受体
定位
消化道
免疫组织化学
大鼠
Gonadotropin releasing hormone receptor(GnRHR)
Localization
Digestive tract
Immunohistochemistry
Rat
