摘要
为了研究促性腺激素释放激素(GnRH)受体在甲壳动物视神经节中的存在及分布情况,采用MaxVisionTM免疫组织化学方法,以兔抗人GnRH受体的多克隆抗体,对不同发育期雌性三疣梭子蟹的视神经节进行了免疫组织化学定位。结果显示,GnRH受体的免疫阳性物质在视神经节的多个部位,视神经层、视外髓、视内髓、视端髓及X器中都有较为广泛的存在,在视外髓、视内髓与X器处的神经分泌细胞中尤为明显。不同发育期的雌性三疣梭子蟹视神经节GnRH受体的免疫阳性分布位置相似,免疫阳性强度存在一定的差异。三疣梭子蟹视神经节存在的GnRH受体免疫阳性物质,为GnRH参与视神经节调节作用提供了形态学依据。
Gonadotropin-releasing hormone （GnRH） is a kind of very important and crucial hormone in neuroregulation and reproduction.In order to elucidate whether the GnRH receptor exists or not,and to investigate its locations in the crustacean-peculiar optic ganglion as well,the immunohistochemical technique of MaxVisionTM method was used in this study.The locations of immunoreactive substances in the optic ganglion of the female swimming crabs,Portunus trituberculatus were found out in different vitellogenesis stages.The results reveal that： the immunoreactive substances of GnRH receptor were widely detected in many parts of the optic ganglion of the female crabs,lamina ganglionaris（LG） ,medulla externa（ME） ,medulla interna（MI） ,medulla terminalis（MT） and X organ as well.And they were more strongly marked in the neurosecretory cells of ME,MI and X organ.In the optic ganglion of the female crabs in different vitellogenesis stages,the locations of immunoreactive substances of GnRH receptor were nearly the same,and there were also differences in the intensity of the immunoreaction.The results of this study,show that the immunoreactive substances of GnRH receptor existing in the optic ganglion of P.trituberculatus,can provide morphologic evidence for GnRH participating in the neuroendocrine regulation of the optic ganglion.
出处
《水产学报》
CAS
CSCD
北大核心
2010年第11期1689-1697,共9页
Journal of Fisheries of China
基金
国家自然科学基金项目(40776084
41076081)
关键词
三疣梭子蟹
GNRH受体
视神经节
免疫组织化学
Portunus trituberculatus
gonadotropin-releasing hormone receptor
optic ganglion
immunohistochemistry
