摘要
目的改进测定血浆中丙戊酸浓度的GC法。方法 HP-FFAP交联弹性石英毛细管柱(0.53 mm×30 m×1.0μm)为色谱柱,FID为检测器,N2为载气,柱温为200℃,以环己烷羧酸为内标,100μl血浆酸化后,用乙酸乙酯萃取后取上清液直接进样。结果丙戊酸在9.8～145.7 mg.L-1范围内线性关系良好,平均回收率为102.7%,日内日间精密度均小于8.5%。应用该方法与氯仿提取经典法,对10名服用丙戊酸类药物患者血浆分析比较,两种方法无统计学差异。结论本法简便、快速、准确,可用于血浆中丙戊酸浓度的快速测定。
Objective To improve the method for the determination of valproic acid in human plasma by GC. Methods Valproic acid was separated and identified on HP-FFAP column （0.53 mm x30 m x 1.0 μn）. The column temperature was 200 ℃. The carrier gas was nitrogen and the detector was FID. The internal standard was cyclohexaneearboxylic acid. Sodium valproate was inverted into valproic acid by adding sulfuric acid to the plasma. The supernatant was injected directly after being extracted with acetic ether. Results The calibration curve of valproic acid was linear over the range of 9.8-145.7 mg .L-1. The average recovery was 102.7%. The intra-day and inter-day precision was both less than 8.5%. There was no statistical difference between the classical chloroform method and the method we improved in analyzing the plasma of ten patients taking valproic acid drugs. Conclusion This method is simple ,quick and accurate. It can be used for the determination of valproic acid in human plasma.
出处
《解放军药学学报》
CAS
2012年第6期511-513,516,共4页
Pharmaceutical Journal of Chinese People's Liberation Army
关键词
GC
丙戊酸
血药浓度
GC
valproic acid
blood drug concentration
